Method for optically determining total hemoglobin concentration

Chemistry: analytical and immunological testing – Hemoglobin – myoglobin – or occult blood

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436 68, 356 41, G01N 2125

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057733015

ABSTRACT:
In a method of optically determining the total hemoglobin concentration (tHb) in non-hemolyzed whole blood, using at least two wavelengths for measuring, a first wavelength of .lambda.<805 nm and a second wavelength of .lambda.>805 nm is employed, such the following is valid for the absorption coefficients of hemoglobin derivatives O.sub.2 Hb and RHb at the two measurement wavelengths .lambda..sub.1 and .lambda..sub.2 : .sigma.O(.lambda..sub.1).about..sigma.R(.lambda..sub.2) and .sigma.R(.lambda..sub.1).about..sigma.O(.lambda..sub.2). The sum of absorption values A.sub.1 and A.sub.2 measured at wavelengths .lambda..sub.1 and .lambda..sub.2 will be a quantity which is proportional to the total hemoglobin concentration tHb and independent of the oxygen saturation O.sub.2sat.

REFERENCES:
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patent: 5692503 (1997-12-01), Kuenstner
S. Takatani et al. "A Miniature Hybrid Reflection Type Optical Sensor . . . Whole Blood" in IEEE, vol. 35, No. 3, Mar. 1988.

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