Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2006-08-22
2006-08-22
McElwain, Elizabeth (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C800S278000, C800S298000, C435S419000
Reexamination Certificate
active
07094952
ABSTRACT:
The subject of the present invention is a method for obtaining transgenic plants expressing a protein with H2O2producing activity, which comprises the following steps of:(a) transforming plant cells withAgrobacterium rhizogenescontaining a vector carrying a gene encoding a protein producing H2O2in a context which allows its expression in the plant;(b) selecting the transformants which contain and express this gene, by a peroxidase-based colorimetric test;(c) regenerating the plants from the roots selected and monitoring the expression of the plantlets obtained by a peroxidase-based colorimetric test;(d) sorting according to phenotype and optionally carrying out the molecular analysis of the progeny of the transgenic plants, allowing the selection or the confirmation of transgenic plants obtained containing only the transgene and not the T-DNA specific toA. rhizogenes.Application: Obtaining of transgenic plants
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Grison Rene
Pagniéz Michel
Toppan Alain
Biogemma
Dennison, Schultz & MacDonald
Helmer Georgia
McElwain Elizabeth
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