Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1987-01-23
1993-05-25
Lacey, David L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
4352402, 43524023, 530351, 530412, C12P 2100, C12N 508, C07K 1506, C07K 316
Patent
active
052139700
ABSTRACT:
Disclosed herein is a novel antitumor factor, termed Human Monocyte Toxin, obtained by the precise activation of cells of monocyte/macrophage lineage. Monocytes are isolated in the absence of endotoxin by counterflow elutriation. HMT release can be triggered by exposure to low levels of 6-0-stearoyl muramyl dipeptide, lipopolysaccharide, phorbol myristate acetate or other known macrophage activating agents. Triggering results in the rapid release of HMT which requires transcription, translation, and intact secretory apparatus. The requirement for precise control of the triggering agent concentration is disclosed.
REFERENCES:
patent: 4495282 (1985-01-01), Ohnishi et al.
patent: 4591557 (1986-05-01), Keyes et al.
patent: 4650674 (1987-03-01), Aggarwal et al.
Kilbourn, "Inhibition of the Mitochondrial Respiration of Tumor Cells by Soluble Factors released by Activated Macrophages", Order #DA8419088, Diss. Abstr. Int. B, 1984, 45(5), 1424-5.
Klostergaard et al., "Effector Mechanisms of Human Monocyte-mediated Tumor Cytotoxicity in Vitro: Biochemical, Functional, and Serological Characterization of Cytotoxins Produced by Peripheral Blood Monocytes Isolated by Counterflow Elutriation", Cancer Res. V46, 2871-2875, Jun. 1986.
Matthews, "Production of an anti-tumor cytotoxin by human monocytes", Dialog/Cancer Lit 1986, Abstract 0304724, Hern/82015985.
Hammerstrom et al, "Soluble Cytostatic Factor(s) Released from Lymphokine-Activated Human Monocytes" Dialog/Cancer Lit 1986 Abstract 0304683 Hern/82015944.
Liang et al, "Human and Mouse Tumor Necrosis Factor Differ in their Reactivity with Neutralizing Antibodies" Dialog/Cancerlit Abstract 1597605 ICDB 86021531, 1986.
Kull et al, "Necrosin: purification and properties of a cytotoxin derived from a murine macrophage-like cell line", PNAS V81, 7932-7936, 1984.
Utushizaki et al, "Definition of tumor-necrosis factor and its production mechanism", Dialog File 154, Medline, 1986.
Liang et al, "Production and Characterization of Monoclonal Antibodies against Recombinant Human Tumor Necrosis Factor cachectin", Biochem. Bio Phys. Res. Commun V137, 847-854, 1986.
Chen et al, "Lipopolysaccharide (LPS) Stimulates Fresh Human Monocytes to lyse Actinomycon D-Treated WEHI-164 Target Cells via increased secretion of a monokine similar to Tumor Necrosis Factor", J. Immunology, V.135(6), 3978-3987, 1985.
Sone et al, "Potentiation of direct antitumor cytoxicity and production of tumor cytolytic factors in human blood monocytes by human recombinant interferon-gamma and muramyl dipeptide derivatives", Abstract, Medline/Dialog 1986.
Gifford et al, "Effects of Rabbit Tumor Necrosis Factor (TNF) On Cells", in Biochemical Characterization of Lymphokines Academic Press, 1980 pp. 307-312.
Aggarwal et al, (A), "Human Tumor Necrosis Factor", in Methods in Enzymology, v. 116, 448-456, 1985.
Klostergaard et al, "Human Monocyte Cytotoxins", Natural Immunity and Biological Response, V4(5), 1985 #256.
Klostergaard et al, (1986), Cancer Res., 46:662-669.
Carroll et al. (1982), Jrnl. Biol. Resp. Mod., 1:245-252.
Unsgaard (1979), Acta path. microbiol. scand., Sect. C, 07:141-149.
Matthews (1981), Immunol, 44:135-142.
Sone et al., (1984), Gann, 75:970-978.
Turpin et al. (1985), Apr. 1985. FASEB Abstract.
Kleinerman et al. (1985), Cancer Res., 45:2058-2064.
Dialog Search Report.
Klostergaard Jim
Lopez-Berestein Gabriel
Turpin Jim
Board of Regents , The University of Texas System
Elliot Geroge C.
Lacey David L.
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