Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Reexamination Certificate
2004-10-19
2008-10-14
Blanchard, David J. (Department: 1643)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
C530S387100, C435S007100, C435S252330, C435S488000, C435S069100, C536S023530
Reexamination Certificate
active
07435804
ABSTRACT:
A bacterial high-expression system which is applicable for simultaneous screening of large numbers of recombinant clones from combinatorial antibody libraries is disclosed. The method pertains to screening of single chain antibodies from libraries expressed in the periplasm ofE. coliby secretion. By this approach, approximately 104clones can be screened in a single round. After screening, the clones, which express the recombinant antibodies to the desired antigen, can be directly used for production of large quantities of antibodies from microorganism culture. The system is especially attractive for fast screening of antibody libraries from a hybridoma source. A refolding method for the large-scale production of biologically active scFv-6 his proteins from bacterial inclusion bodies is also disclosed.
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Deryabina Olena
Gilchuk Pavlo
Irodov Dmitro
Kordyum Vitaliy A.
Okunev Oleg
Blanchard David J.
Bristol Lynn
Knobbe Martens Olson & Bear LLP
Phage Biotechnology, Inc.
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