Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-12-15
1999-06-08
Railey, II, Johnny F.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 71, 435 772, 435325, 435350, 435357, 435363, 435366, C12N 510, C12N 1563, C12N 1567
Patent
active
059104341
ABSTRACT:
This invention provides a method for obtaining a recombinant retroviral packaging cell capable of producing retroviral vectors and the recombinant packaging cell obtained by the method. Also provided is a method of producing recombinant retroviral particles obtained by introducing into the packaging cells obtained according to the methods disclosed herein, a recombinant retroviral vector and propagating the resulting producer cells under conditions favorable for the production and secretion of retroviral vector supernatant. The retroviral supernatants produced by these methods also is claimed herein. This invention further provides a method for screening retroviral vector supernatant for high transduction efficiency and methods for producing retroviral vector supernatant for transducing cells with high efficiency in gene therapy applications.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4754065 (1988-06-01), Levenson et al.
patent: 4800159 (1989-01-01), Mullis et al.
patent: 5061620 (1991-10-01), Tsukamoto et al.
patent: 5278056 (1994-01-01), Bank et al.
patent: 5498537 (1996-03-01), Bresler et al.
patent: 5591624 (1997-01-01), Barber et al.
patent: 5686279 (1997-11-01), Finer et al.
patent: 5716826 (1998-02-01), Gruber et al.
Delouis, et al., "Xenotropic and amphotropic pseudotyped recombinant retrovirus to transfer genes into cells from various species" Biochem. Biophys. Res. Commun. (1990) 169(1):8-14.
DeMonte, et al., "Gene transfer by retrovirus-derived shuttle vectors in the generation of murine bispecific monoclonal antibodies" Proc. Natl. Acad. Sci. (1990) 87(8):2941-2945.
Dougherty, et al., "New Retrovirus Helper Cells with Almost No Nucleotide Sequence Homology to Retrovirus Vectors" J. Virol. (1989) 63(7):3209-3212.
Gelinas, et al. "Retroviral vectors for the beta-globin gene that demonstrates improved titer and expression" Ann. N.Y. Acad. Sci. (1990) 612:427-441.
Lehn, P.M., "Gene therapy using bone marrow transplantation: a 1990 update" Bone Marrow Transplant (1990) 5(5):287-293.
Muenchau, et al., "Analysis of retroviral packaging lines for generation of replication-competent virus" Virology (1990) 176(1):262-265.
Sorge, et al., "Amphotropic retrovirus vector system for human cell gene transfer" Mol. Cell. Biol. (1984) 4(9):1730-1737.
Markowitz et al., Journal of Virology 62(4):1120-1124 (1988).
Markowitz et al., Virology 167(2):400-406 (1988).
Bagnis et al., "Leukemogenicity of v-myb-transformed monoblasts cells can be modulated by normal bone marrow environment" Oncogene (1993) 8:737-743.
Bevec et al., "Inhibition of human immunodeficiency virus type 1 replication in human T cells by retroviral-mediated gene transfer of a dominant-negative Rev trans-activator" Proc. Natl. Acad. Sci. USA (1992) 89:9870-9874.
Bonnerot et al., "A .beta.-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies" Proc. Natl. Acad. Sci. USA (1987) 84:6795-6799.
Burns et al., "Vesicular stomatitis virus G glycoprotein pseudotyped retroviral vectors: Concentration to very high titer and efficient gene transfer into mammalian and nonmammalian cells" Proc. Natl. Acad. Sc. USA (1993) 90:8033-8037.
Cornetta et al., "Infection of human cells with murine amphotropic replication-competent retroviruses" Human Gene Therapy (1993) 4:579-588.
Cosset et al., "High-titer packaging cells producing recombinant retroviruses resistant to human serum" J. Virol. (1995) 69(12):7430-7436.
Cosset et al., "Retroviral retargeting by envelopes expressing an N-terminal binding domain" J. Virol. (1995) 69(10):6314-6322.
Cosset et al., "Use of helper cells with two host ranges to generate high-titer retroviral vectors" Virology (1993) 193:385-395.
Danos et al., "Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges" Proc. Natl. Acad. Sci. USA (1988) 85:6460-6464.
Dranoff et al., "Vaccination with irradiated tumor cells engineered to secrete murine granulocyte-macrophage colony-stimulating factor stimulates potent, specific, and long-lasting anti-tumor immunity" Proc. Natl. Acad. Sci. USA (1993) 90:3539-3543.
Escaich et al., "RevM10-mediated inhibition of HIV-1 replication in chronically infected T cells" Human Gene Therapy (1995) 6:625-634.
Evans et al., "A neutralizable epitope common to the envelope glycoproteins of ecotropic, polytropic, xenotropic, and emphotropic murine leukemia viruses" J. Virol. (1990) 64(12):6176-6183.
Finer et al., "kat: A high efficiency retroviral transduction system for primary human T lymphocytes" Blood (1994) 83(1):43-50.
Forestell et al., "Improved detection of replication-competentretrovirus" J. Virol. Meth. (1996) 60:171-178.
Forestell et al., "Retroviral end-point titer is not predictive of gene transfer efficiency: implications for vector production" Gene Therapy (1995) 2:723-730.
Gilboa, "Retroviral gene transfer: Applications to human therapy" Adv. Exp. Med. Biol. (1988) 241:29-33.
"Gene Expression Technology" Methods in Enzymology (1991) Goeddel, et al. eds., Academic Press, Inc. New York. The title page and table of contents are enclosed herewith.
Haapala et al., "Isolation from cats of an endogenous type C virus with a novel envelope glycoprotein" J. Virol. (1985) 53(3):827-833.
Hoatlin et al., "Amplified and tissue-directed expression of retroviral vectors using ping-pong techniques" J. Mol.Med. (1995) 73:113-120.
Hodgson, "Expression Systems: A user's guide" Bio/Techniques (1993) 11:887-893.
Irving et al., "A reverse transcriptase-polymerase chain reaction assay for the detection and quantitation of murine retroviruses" Bio/Technol. (1993) 11:1042-1046.
Joshi et al., "Reduction in growth temperature minimizes instability of large plasmids containing HIV-1 proviral genomes" BioTechniques (1993) 14:880-886.
Kantoff et al., "Expression of human adenosine deaminase in nonhuman primates after retrovirus-mediated gene transfer" J. Exp. Med. (1987) 166:219-234.
Kasahara et al., "Tissue-specific targeting of retroviral vectors through ligand-receptor interactions" Science (1994) 266:1373-1376.
Kotani et al., "Improved methods of retroviral vector transduction and production for gene therapy" Human Gene Therapy (1994) 5:19-28.
Kozak, "At least six nucleotides preceding the AUG initiator codon enhance translation in mammalian cells" J. Mol. Biol. (1987) 196:947-950.
Lander et al., "A Mus dunni cell line that lacks sequences closely related to endogenous murine leukemia viruses and can be infected by ecotropic, amphotropic, xenotropic, and mink cell focus-forming viruses" J. Virol. (1984) 52(2):695-698.
Luskey et al., "Gene transfer into murine hematopoietic stem cells and bone marrow stromal cells" Ann. N.Y. Acad. Sci. (1990) 612:398-406.
Markowitz et al., "A safe packaging line for gene transfer: Separating viral genes on two different plasmids" J. Virol. (1988) 62(4):1120-1124.
Markowitz et al., "Construction and use of a safe and efficient amphotropic packaging cell line" Virology (1988) 167:400-406.
Marsh et al., "Retention of progenitor cell function in CD34.sup.+ cells purified using a novel O-sialoglycoprotease" Leukemia (1992) 6(9):926-934.
Miller et al., "Improved retroviral vectors for gene transfer and expression" BioTechniques (1989) 7(9):980-990.
Miller et al., "Redesign of retrovirus packaging cell lines to avoid recombination leading to helper virus production" Mol. Cell Biol. (1986) 6(8):2895-2902.
Miller et al., "Two base changes restore infectivity to a noninfectious molecular clone of moloney murine leukemia virus (pMLV-1)" J. Virol. (1984) 49:214-222.
Miller, "Progress toward human gene therapy" Blood (1990) 76(2):271-278.
Miller, "Retrovirus packaging cells" Human Gene Therapy (1990) 1:5-14.
Morgenstern et al., "Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line" Nucl. Acids. Res. (1990) 18(12):3587-3596.
"Orda Reports. Recombinant DNA Advisory Committee (RAC). Data Management Report Jun. 1994" Human Gene Therapy (1994) 5:1293-1302
Bohnlein Ernst
Chen Jingyi
Dando Jonathan S.
Forestell Sean P.
Plavec Ivan
Railey II Johnny F.
Systemix, Inc.
LandOfFree
Method for obtaining retroviral packaging cell lines producing h does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for obtaining retroviral packaging cell lines producing h, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for obtaining retroviral packaging cell lines producing h will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1683040