Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1986-03-27
1988-07-12
Weimar, Elizabeth
Chemistry: molecular biology and microbiology
Spore forming or isolating process
43524023, 435948, C12N 500, C12R 191
Patent
active
047570199
ABSTRACT:
Human melanocyte cells are grown in tissue culture medium containing fibroblast growth suppressor plus tumor growth promoters at pH 7.2 to about 7.4. In a stepwise procedure, Langerhans, keratinocytes and most fibroblasts are removed in the presence of the above suppressor and promoter followed by a subsequent trypsinization treatment. In a final purification step, remaining fibroblasts are separated from the melanocytes by reacting the cell mixture with monoclonal antibody specific for melanocytes, and then separating out the melanocytes from the fibroblasts on density gradients.
REFERENCES:
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Eisinger Magdalena
Marko Olga
Sloan-Kettering Institute for Cancer Research
Weimar Elizabeth
White John P.
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