Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Lyase
Patent
1995-06-06
1997-01-21
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Lyase
435183, 435814, 530350, C12N 988, C12N 900, C07K 114
Patent
active
055959016
ABSTRACT:
A method for preparing an active and inactive adenylate cyclase from a culture of Bordetella parapertussis having specific reactivity with polyclonal or monoclonal antibodies to adenylate cyclase from Bordetella pertussis is presented. The inactive adenylate cyclase is devoid of calmodulin-activatable adenylate cyclase activity and of affinity for calmodulin. The method comprises culturing a clone of Bordetella parapertussis, homogenizing the culture to produce a homogenate, and isolating the active and inactive adenylate cyclase from the homogenate by urea precipitation. The isolated active and inactive adenylate cyclase may be used to prepare a vaccine for the prevention of Bordetella infection.
REFERENCES:
patent: 5095094 (1992-03-01), Brezin et al.
patent: 5312749 (1994-05-01), Griffin et al.
Brennan et al. (1988), Infect. Immun., 56(12), "Identification of a 69-Kilodalton Nonfimbial Protein As an Agglutiongen of Bordetella pertussis", pp. 3189-3195.
Charles et al. (1989) Proc. Nat. Acad. Sci., USA, 86, "Molecular Cloning and Characterization of Protective Outer Membrane Protein P.69 from Bordetella pertussis", pp. 3554-3558.
Novotny et al. (1991) J. Infect. Disease, 164, "Biologic and Protective Properties of the 69-kDa Outer Membrane Protein from Bordetella pertussis: A Novel Formulation for an Acellular Pertussis Vaccine", pp. 114-122.
Pittman (1974) Bergey's Manual of Determinative Bacteriology, Eighth Edn., Buchanan et al., Eds., Williams & Wilkins, Baltimore, pp. 282-283.
Weiss (1992) The Prokaryotes, Second Edn., Balows et al., Eds., Springer-Verlag, New York, pp. 2530-2543.
"Bordetella pertussis Adenylate Cyclase, Identification of Multiple Forms of the Enzyme by Antibodies," Rogel et al., The Journal of Biological Chemistry, vol. 263, No. 26, pp. 13310-13316, Sep. 13, 1988.
"Adenylate Cyclase Toxin from Bordetella pertussis, Identification and Purification of the Holotoxin Molecule," Hewlett et al., The Journal of Biological Chemistry, vol. 264, No. 32, pp. 19379-19384, Nov. 15, 1989.
"Bordetella pertussis Adenylate Cyclase: Isolation and Purification by Calmodulin-Sepharose 4B Chromatography," Friedman, R. L., Infection and Immunity, vol. 55, No. 1, pp. 129-134, Jan. 1987.
"Secreted Adenylate Cyclase of Bordetella pertussis: Calmodulin Requirements and Partial Purification of Two Forms," Kessin et al., Journal of Bacteriology, vol. 166, No. 1, pp. 290-296, Apr. 1986.
"Bordetella pertussis Adenylate Cyclase Purification, Characterization and Radioimmunoassay," Ladant et al., The Journal of Biological Chemistry, vol. 261, No. 34, pp. 16264`16269, Dec. 5, 1986.
"Purification and Characterization of a Calmodulin-Sensitive Adenylate Cyclase from Bordetella pertussis," Shattuck et al., Biochemistry, vol. 24, No. 23, pp. 6356-6362, 1985.
"Bordetella Adenylate Cyclase: A Genus Specific Protective Antigen and Virulence Factor," Novotny et al., Proceedings of the Fourth International Symposium on Pertussis, Develop. biol. Standard., vol. 61, pp. 27-41 (S. Karger, Basel, 1985).
"Adenyl Cyclase in Bordetella pertussis Vaccines," Hewlett et al., Journal of Infectious Diseases, vol. 36, Suppl., pp. S216-S219, 1977.
"Adenylate Cyclase Activity of a 68,000-Molecular-Weight Protein Isolated from the Outer Membrane of Bordetella bronchiseptica," Novotny et al., Infection and Immunity, vol. 50, No. 1, pp. 199-206, Oct. 1985.
Chemical Abstracts, vol. 105: abstract No. 186455s (1986), "Bordetella pertussis adenylate cyclase. Purification, characterization, and radioimmunoassay."5
Finnegan, Henderson, Farabow, Garrett and Dunner, L.L.P.
Alonso Jean M.
Boucaud Jean L.
Brezin Colette
Rocancourt Murielle
Szatanik Marek
Institut National de la Sante et de la Recherche Medicale
Institut Pasteur
Weber Jon P.
Wityshyn Michael G.
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