Method for obtaining periplasmic proteins from bacterial cells u

Details Method for obtaining periplasmic proteins from bacterial cells u Method for obtaining periplasmic proteins from bacterial cells u

1984-04-03

1985-02-05

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

435 68, 435879, 426656, C07G 700, A23J 118

Patent

active

044977301

ABSTRACT:
The present invention discloses a method of extracting periplasmic protein comprising treating a cellular suspension of an organism possessing said periplasmic proteins with a periplasmic protein releasing volume of chloroform for a sufficient time to release said protein and then separating said protein from said cellular suspension.

REFERENCES:
patent: 3983008 (1976-09-01), Shinozaki et al.
patent: 4435386 (1984-03-01), Ribi et al.
patent: 4448714 (1984-05-01), Cunliffe et al.
patent: 4451446 (1984-05-01), Vandevelde et al.
Nossal and Heppel, J. Biol. Chem., 3055, 1966.
Hogg, J. Bacteriol., 105, 604, 1971.
Ames et al., Biochem., 15: 616, 1976.

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