Method for obtaining of glucose isomerase

Details Method for obtaining of glucose isomerase Method for obtaining of glucose isomerase

1980-12-03

1982-03-02

Shapiro, Lionel M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Isomerase

435886, C12N 992

Patent

active

043178839

ABSTRACT:
A method for producing glucose isomerase comprising the steps of cultivating the enzyme producing strain Streptomyces sp. 1339 registration N. 144 Bulgarian State Institute for Drug Control, for 36 to 72 hours in a culture medium with xylose as an indicator, the temperature being kept at 24.degree. to 36.degree. C., the initial pH of cultivation being from 6.5 to 9.0 and effective isomerization at a temperature of 50.degree. to 80.degree. C., and a pH of 6.0 to 9.0 in the presence of 1.times.10.sup.-4 M CoCl.sub.2.6H.sub.2 O 1.times.10.sup.-2 M MgSO.sub.4.7H.sub.2 O in 0.1 to 3 M of the substrate.

REFERENCES:
patent: 3826714 (1974-07-01), Suekane et al.
patent: 3956066 (1976-05-01), Coker et al.
patent: 3979261 (1976-09-01), Outtrup
patent: 4061539 (1977-12-01), Lee
patent: 4255521 (1981-03-01), Hirohara et al.
patent: 4283496 (1981-08-01), Lee

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