Method for obtaining nucleic acids from an environment...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S069100

Reexamination Certificate

active

10148328

ABSTRACT:
The invention concerns a method for preparing nucleic acids from an environment sample, more particularly a method for obtaining a library of nucleic acids from a sample. The invention also concerns nucleic acids of nucleic acid libraries obtained by said method their use in the synthesis of novel compounds, in particular novel compounds of therapeutic interest. The invent further concerns novel means used in the method for obtaining said nucleic acids, such as novel vectors and novel processes for preparing such vectors or recombinant host cells containing said nucleic acid. Finally, the invention concerns methods for detecting a nucleic acid of interest within a library of nucleic acids resulting from said method, and nucleic acids detected by said method and polypeptides encoded by said nucleic acids.

REFERENCES:
patent: 4900677 (1990-02-01), Hewitt
patent: 5688689 (1997-11-01), Smokvina et al.
patent: 5824485 (1998-10-01), Thompson et al.
patent: WO 96/34112 (1996-10-01), None
patent: WO96/34112 (1996-10-01), None
patent: WO 99/20799 (1999-04-01), None
patent: WO 99/67374 (1999-12-01), None
Jacobsen CS and Rasmussen OF, “Development and application of a new method to extract bacterial DNA from soil based on separation of bacteria from soil with cation-exchange resin”, Applied and Environmental 6Microbiology, 1992, p. 2458-62.
Simonet P, et al. “Identification of Frankia strains in nodules by hybridization of polymerase chain reaction products with strain-specific oligonucleotide probes”, 1990, Arch Microbiol, 153: 235-240.
Ezaki, T. et al. “Achromopeptidase for lysis of anaerobic gram-positive cocci”, J of Clin Micro. 1982, p. 844-846.
Higuchi, R, et al. “A general method for cloning eukaryotic structural gene sequences”, PNAS, 1976, vol. 73, p. 3146-3150.
Ullrich, A. et al. “Rat insulin genes: construction of plasmids containing the coding sequences”, 1977, 196(4296), p. 1313-1319.
Bakken, LR, “Separation and purification of bacteria from soil”, Applied and Environmental Microbiology, 1985, p. 1482-1487.
Steffan RJ, et al. “Recovery of DNA from soils and sediments”, Applied and Environmental Microbiology, 1988, p. 2908-2915.
Nesme, H et al. “Specific DNA Sequences for detection of Soil Bacteria”, Nucleic Acids in the Environment (Trevors, et al. Eds), Springer-Verlag, Heidelberg, 1995, p. 111-139.
Seow, K-T, et al. “A study of iterative Type II polyketide synthases: using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms” Journal of Bacteriology, 1997, p. 7360-7368.
Nesme, H et al. “Specific DNA sequences for detection of Soil Bacteria”, Nucleic Acids in the Environment (Trevors, et al., Eds), Springer-Verlag, Heidelberg, 1995, p. 111-139.
Amann et al., Phylogenetic Identification and in Situ Detection of Individual Microbial Cells without Cultivation,Microbiol. Rev., 59:143-169, 1995.
Bakken, Separation and Purification of Bacteria from Soil,Appl. Environ. Microbiol., 49: 1482-1487, 1985.
Clegg et al., Direct Extraction of Microbial Community DNA from Humified Upland Soils,Lett. Appl. Microbiol., 25: 30-33, 1997.
Clerc et al., Efficiency of The Transfer of a pSAM2-Derivative Plasmid between Two Strains ofStreptomyces lividansin Conditions Ranging from Agar Slants to Non-Serile Soil Microcosms,FEMS Microbilogy Ecology, 21: 157-165, 1996.
Frostegärd et al., Microbial Biomass Measured as Total Lipid Phosphate In Soils of Different Organic Content,J. Microbiol. Meth., 14: 151-163, 1991.
Frostegärd et al., Quantification of Bias Related to The Extraction of DNA Directly from Soils,Appl. Environ. Microbiol., 65: 5409-5420, 1999.
Fu et al., Antibiotic Activity of Polyketide Products Derived from Combinatorial Biosynthesis: Implications for Directed Evolution,Molecular Diversity, 1: 121-124, 1995.
Holben et al., DNA Probe Method for the Detection of Specific Microorganisms in The Soil Bacterial Community,Appl. Environ. Microbiol., 54: 703-711, 1988.
Jacobsen et al., Development and Application of a New Method To Extract Bacterial DNA from Soil Based on Separation of Bacteria from Soil with Cation-Exchange Resin,Appl. Environ. Microbiol., 58: 2458-2462, 1992.
Kuske et al., Small-Scale DNA Sample Preparation Method for Field PCR Detection of Microbial Cells and Spores in Soil,Appl. Environ. Microbiol., 64: 2463-2472, 1998.
Leff et al., Comparison of Methods of DNA Extraction from Stream Sediments,Appl. Environ. Microbiol., 61: 1141-1143, 1995.
Liesack et al., Occurrence of Novel Groups of The Domain Bacteria as Revealed by Analysis of Genetic Material Isolated from An Australian Terrestrial Environment,J. Bacteriol., 174: 5072-5078, 1992.
Liesack et al., Microbial Diversity in Soil: The Need for A Combined Approach Using Moleular and Cultivation Techniques,in Modern Soil Microbiology(van Elsas et al., Eds.), Marcel Dekker, Inc., New York, 1997, pp. 375-439.
Maidak et al., A New Version of the RDP (Ribosomal Database Project),Nucleic Acids Research, 27: 171-173, 1999.
Miller et al., Evaluation and Optimization of DNA Extraction and Purification Procedures for Soil and Ediment Samples,Appl. Environ. Microbiol., 65: 4715-4724, 1999.
Moré et al., Quantitative Cell Lysis of Indigenous Microorganisms and Rapid Extraction of Microbial DNA from Sediment,Appl. Environ. Microbiol., 60: 1572-1580, 1994.
Nesme et al., Specific DNA Sequences for Detection of Soil Bacteria,inNucleic Acids in the Environment, (Trevors et al., Eds.), Springer-Verlag, Heidelberg, 1995, pp. 111-139.
Ogram et al., Effects of DNA Polymer Length on Its Adsorption to Soils,Appl. Environ. Microbiol., 60: 393-396, 1994.
Ogram et al., The Extraction and Purification of Microbial DNA from Sediments,J. Microbiol. Methods, 7: 57-66, 1987.
Paget et al., Adsorption of DNA on Clay Minerals: Protection against DNasel and Influence on Gene Transfer,FEMS Microbiol. Lett., 97: 31-40, 1992.
Paget et al., The Fate of Recombinant Plant DNA in Soil,Eur. J. Soil. Biol., 34: 81-88, 1998.
Picard et al., Detection and Enumeration of Bacteria in Soil by Direct DNA Extraction and Polymerase Chain Reaction,Appl. Environ. Microbiol., 58: 2717-2722, 1992.
Raynal et al. Structure of The Chromosomal Insertion Site for pSAM2: Function Analysis inEscherichia coli, Molecular Microbiology, 28: 333-342, 1998.
Seow et al., A Study of lterative Type II Polyketide Synthases, Using Bacterial Genes Cloned from Soil DNA: A Means to Access and Use Genes from Uncultured Microorganisms,J. Bacteriol., 179: 7360-7368, 1997.
Smalla et al., Rapid DNA Extraction Protocol from Soil for Polymerase Chain Reaction-Mediated Amplification,J. Appl. Bacteriol, 74: 78-85, 1993.
Stein et al., Characterization of Uncultivated Prokaryotes: Isolation and Analysis of A 40-Kilobase-Pair Genome Fragment from A Planktonic Marine Archaeon,J. Bacteriol., 178: 591-599, 1996.
Steffan et al., Recovery of DNA from Soils and Sediments,Appl. Environ. Microbiol., 54: 2908-2915, 1988.
Tebbe et al., Interference of Humic Acids and DNA Extracted Directly from Soil in Detection and Transformation of Recombinant DNA from Bacteria and A Yeast,Appl. Environ. Microbiol.,59: 2657-2665, 1993.
Torsvik, Isolation of Bacterial DNA from Soil,Soil Biol. Biochem., 12: 15-21, 1980.
Tsai et al., Rapid Method for Direct Extraction of DNA from Soil and Sediments,Appl. Environ. Microbiol., 57: 1070-1074, 1991.
van Elsas et al., Microbiological and Molecular Biological Methods for Monitoring Microbial Inoculants and Their Effects in The Soil Environment,J. Microbiol. Methods, 32: 133-154, 1998.
van Elsas et al., Soil DNA Extraction and Assessment of The Fate of Mycobacterium Chlorophenolicum Strain PCP-1 in Different Soils by 16S Ribosomal RNA Gene Sequence Based Most-Probable-Number PCR and Immunofluorescence,Biol. Fertl. Soils, 24: 188-195, 1997.
Volossiouk et al., Direct DNA Extraction for PCR-Mediated Assays of Soil Organisms,Appl. Environ. Microbiol., 61: 3972-3976, 1995.
Ward, et al., 16S rRNA Sequences

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