Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Reexamination Certificate
2011-03-29
2011-03-29
Hobbs, Lisa J (Department: 1657)
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
C435S219000, C435S183000
Reexamination Certificate
active
07915039
ABSTRACT:
The invention relates to the field of cell biology. In detail it relates to the obtainment of mesenchymal stem cells from human tissue. This invention might be applicable in healing within the frame of the treatment of several diseases.Due to the invention it will be possible to obtain mesenchymal stem cells from human tissue with high homogeneity of the cell suspension, since the used method for obtaining mesenchymal stem cells from human tissue comprises the crushing and enzymatical treatment of the tissue with collagenase solution in Eagle medium in the Dulbecco modification, removal of erythrocytes by the aid of the lysis solution and subsequent filtration of the prepared suspension; as human tissue fat tissue or decidual or amniotic placenta membrane or chorion placenta stroma is used, whereas the filtration is performed sequentially by the use of filters comprising a pore size of 100 μm and 10 μm. In the enzymatical treatment of the fat tissue, of the decidual or amniotic placenta membrane collagenase of the type I is used, and in the enzymatical treatment of the chorion placenta stroma collagenase of the type IV is used.
REFERENCES:
patent: 5842477 (1998-12-01), Naughton et al.
patent: 2003/0161817 (2003-08-01), Young et al.
patent: 2003/0235563 (2003-12-01), Strom et al.
Campagnoli et al., Blood (2001) 98:2396-2402.
Fridenshtein et al., Exp. Hematol. (1974) 2:83-92.
Grontos et al., Blood (1995) 85:929-940.
Heynesworth et al., Bone (1995) 13:81-85.
Majumdar et al., J. Cell. Physiol. (2000) 185:98-106.
Rao et al., Mech. Ageing Dev. (2001) 122:713-734.
Romanov et at., Stem Cells. (2003) 21:105-110.
Zuk et al., Molecular Biology of the Cell (2002) 13:4279-4295.
Zuk et al. (2001) Multilineage Cells from Human Adipose Tissue: Implications for Cell-Based Therapies. Tissue Engineering 7(2):211-228.
Hobbs Lisa J
Klarquist & Sparkman, LLP
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