Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-05-16
1997-05-06
Sisson, Bradley L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435810, 436 94, 536 231, 536 243, 536 2431, 536 2432, 935 77, 935 78, C12Q 168, H01L 2120, C07H 2102, C12N 1500
Patent
active
056270291
ABSTRACT:
The subject invention relates to a method for processing a liquid sample of biological material by: a) transferring a liquid sample containing both uncultured informative cells and non-informative cells onto a substrate wherein the surface tension of the liquid sample is sufficient to maintain the liquid sample beaded on the surface of the substrate; b) incubating the beaded liquid sample on the surface of the substrate for a period of time appropriate for the selective settling of the informative cells onto the surface of the substrate; and c) breaking the surface tension of the liquid portion of the sample wherein the liquid portion of the sample is removed along with the non-informative cells and wherein the informative cells remain on the surface of the substrate.
A kit for performing the method is also provided.
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Burns, J. et al. (1985) "Sensitive system for visualising biotinylated DNA probes hybridised in situ: rapid sex determination of intact cells" J Clin Pathol 38:1085-1092.
Pardue, M. (1985) "In Situ Hybridisation".
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Rajendra, B.R. et al. (1980) "A New and Simple Technique for Chromosomal Preparations from Peripheral Blood Lymphocytes, Amniotic Cell Cultures, Skin Fibroblasts, Bone Marrow and Single Cell Clones when the Yields form Harvests are Low" Human. Genet., 55:363-366.
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Dackowski William R.
Houseal Timothy W.
Klinger Katherine W.
Pavelka Karen
Genzyme Corporation
Sisson Bradley L.
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