Method for obtaining human hepatocyte cultures

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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43524021, C12N 508

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active

051127571

ABSTRACT:
A method for culturing human hepatocytes is disclosed. The method comprises associating a culture of human hepatocytes including a medium with cells of hepatic origin, these cells being different from the hepatocytes and being of the type insuring in vivo specific cellular interactions with the hepatocytes. The culture obtained maintains hepatocyte functions at a high level for an extended period of time.

REFERENCES:
patent: 3935066 (1976-01-01), Apostolov
News article (France) "La voie des cellules de culture" Newspaper: La Vie Scientifique, Jan. 30, 1987.
Baffet et al., Biochem. Biophys. Res. Comm. 109: 507-512, (1982).
Guillouzo et al., Triangle 20: 121-128, (1981).
Guguen-Guillouzo et al., Exp. Cell Res. 143:47-54, (1983).

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