Method for obtaining highly pure beta 1,3-glucan from euglena

Details Method for obtaining highly pure beta 1,3-glucan from euglena Method for obtaining highly pure beta 1,3-glucan from euglena

1993-03-22

1995-01-31

Naff, David M.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

4352571, 4352574, 435262, 435946, 47 14, C12P 1904, D06M 1600, A01G 700

Patent

active

053858328

ABSTRACT:
Substantially pure, pyrogen-free beta-1,3-glucan is produced by cultivating Euglena cells in a defined growth medium and under specified conditions that provide a cell mass comprising 70% to 90% beta-1,3-glucan on a dry weight basis, separating the cell mass from the supernatant, extracting the cell mass with methanol and chloroform, acid-washing the extracted cell mass, and washing the acid-treated material with water.

REFERENCES:
patent: 4745058 (1988-05-01), Townsley
Patent Abstracts of Japan entitled "Production of .beta.-1,3-glucan" (24 May 1989) vol. 13, No. 224, (C-599) [3572] & Japanese Patent Application No. 137297 (7 Feb. 1989).
Patent Abstracts of Japan entitled "Production of .beta.-1,3-glucan by cell of genus Euglena" (15 Aug. 1988) vol. 12, No. 299, (C-520) [3146] & Japanese Patent Application No. 6371192 (31 Mar. 1988).
Patent Abstracts of Japan entitled "Culturing of single-celled green algae" (18 Jul. 1981) vol. 5, No. 111, (C-63) [783] & Japanese Patent Application No. 5648883 (2 May 1981).
Di Luzio, "Immunopharmacology of glucan: a broad spectrum enhancer of host defense mechanisms" Trends in Pharmaceutical Science (1983) 4:344-347.
Tomos et al., "A protein-glucan intermediate during paramylon synthesis" Biochem. J. (1978) 174:283-290.
Wolken et al., "Euglena: an experimental organism for biochemical and biophysical studies" Institute of Microbiology, Rutgers, The State University, (1961) pp. 13.
Dwyer et al. (1971) Aust. J. Biol. Sci. 24:15-22.
Prasada et al. (1984) Phytochemistry 23:2531-2534.
Kiss et al., (1986) J. of Phycology 22:327-333.
Invi et al. (1988) Agric Biol Chem 52:49-54.

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