Method for obtaining G protein-coupled receptor (GPCR)...

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C530S388220, C023S300000

Reexamination Certificate

active

07947807

ABSTRACT:
An antibody that specifically binds a three dimensional epitope on the IC3 loop of a GPCR is provided. The antibody may be employed in a method that comprises: contacting a GPCR with a monovalent version of the antibody binding conditions to form a complex; and crystallizing the complex.

REFERENCES:
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Baker, M., 2010, Making membrane proteins for structures: a trillion tiny tweaks, Nat. Meth. 7(6):429-434.
Rosenbaum, D. M., et al., 2009, The structure and function of G-protein-coupled receptors, Nature 459:356-363.
Chayen, N. E., and E. Saridakis, 2008, Protein crystallization: from purified protein to diffraction-quality crystal, Nat. Meth. 5(2):147-153.
Mancia; et al., “Production and characterization of monoclonal antibodies sensitive to conformation in the 5HT2c serotonin receptor”, PNAS, Mar. 13, 2007, 104(11):4303-4308.
Yohannan; et al., “The evolution of transmembrane helix kinks and the structural diversity of G protein-coupled receptors”PNAS, Jan. 27, 2004, 101(4):959-963.
Day; et al., “A monoclonal antibody for G protein-coupled receptor crystallography”, Nat Methods, 2007 Nov. 4 (11)927-9.
Kobilka; et al., “G protein coupled receptor structure and activation”, Biochim Biophys Acta, 2007 Apr. 1768(4) 794-807.
Whorton; et al., A monomeric G protein-coupled receptor isolated in a high-density lipoprotein particle efficiently activates its G protein.

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