Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...
Patent
1987-09-16
1989-03-21
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Blood proteins or globulins, e.g., proteoglycans, platelet...
435 68, 435 69, 530387, 424 85, A61K 39395
Patent
active
048144339
ABSTRACT:
A method for obtaining a papain-free F(ab').sub.2 antibody fragment preparation derived from papain digested whole IgG antibody employing an antibody to papain for binding to and removing papain-associated digestion products from the preparation. The method is particularly useful for digesting whole IgG antibodies which are otherwise sensitive to pepsin digestion to provide F(ab').sub.2 antibody fragments from which Fab' antibody fragments can be obtained having available free sulfhydryl groups in the hinge region.
REFERENCES:
patent: 4479934 (1984-10-01), Sedlarek et al.
patent: 4719107 (1988-01-01), Carosella et al.
patent: 4742159 (1988-05-01), Batz et al.
Biochem. J., 73:(1959), 119-126, Porter.
J. Immunol. Methods, 53 (1982), 133-173, Parham et al.
Collins Daniel W.
Miles Inc.
Schain Howard E.
LandOfFree
Method for obtaining a papain-free antibody fragment preparation does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for obtaining a papain-free antibody fragment preparation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for obtaining a papain-free antibody fragment preparation will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-477838