Method for obtaining a papain-free antibody fragment preparation

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Blood proteins or globulins – e.g. – proteoglycans – platelet...

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435 68, 435 69, 530387, 424 85, A61K 39395

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048144339

ABSTRACT:
A method for obtaining a papain-free F(ab').sub.2 antibody fragment preparation derived from papain digested whole IgG antibody employing an antibody to papain for binding to and removing papain-associated digestion products from the preparation. The method is particularly useful for digesting whole IgG antibodies which are otherwise sensitive to pepsin digestion to provide F(ab').sub.2 antibody fragments from which Fab' antibody fragments can be obtained having available free sulfhydryl groups in the hinge region.

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patent: 4742159 (1988-05-01), Batz et al.
Biochem. J., 73:(1959), 119-126, Porter.
J. Immunol. Methods, 53 (1982), 133-173, Parham et al.

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