Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-05-19
1998-11-03
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
536 232, 435232, 4352523, 43525233, 4353201, C12N 1510, C12N 1560, C12N 988
Patent
active
058307265
ABSTRACT:
The cloning of the heparinase gene from Flavobacterium Heparinum using the polymerase chain reaction is described. The Open Reading Frame (ORF) corresponded to 1152 base pairs encoding a precursor protein of MW 43,800 daltons. The amino acid sequence reveals a 20-residue leader peptide. The gene was expressed in two expression systems in E. coli.
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V.C. Yang et al., "Purification and Characterization of Heparinase From Flavobacterium heparinum", J. Biol. Chem. 260(3): 1849-1857, Feb. 1985.
S.L. Berger et al. (eds.) "Guide to Molecular Cloning Techniques", Meth. Enzymol. 152: 393-399, 415-423, 432-447, 661-704, 1987.
M.P. Deutscher (ed), "Guide to Protein Purification", Meth. Enzymol. 182: 602-613, 738-751, 1991.
Cooney Charles I.
Langer Robert S.
Moremen Kelley
Sasisekharan Ramnath
Zimmermann Joseph J.
Massachusetts Institute of Technology
Prouty Rebecca E.
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