Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-10-18
1996-08-13
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 9121, 435 915, 536 221, 536 231, C12P 1934, C07H 2100
Patent
active
055455395
ABSTRACT:
This invention relates to an improvement of the procedure for amplifying a target nucleotide sequence by using an effective amount of a glycine-based osmolyte in the reaction mixture of an amplification procedure. It has been found that the use of a glycine-based osmolyte reduces the appearance of stutter bands in the amplification product allowing for easier detection of the target nucleotide sequence. For example, detection of the target trinucleotide repeat sequence, indicative of Huntington's Disease, is made clearer with the use of a glycine-based osmolyte.
REFERENCES:
patent: 5192659 (1993-03-01), Simons
patent: 5386024 (1995-01-01), Kacian et al.
Han, Jian et al. (1994), "Over Representation of the disease associated (CAG) and (CGG) repeats in the human genome", Nucleic Acids Research vol. 22, No. 9 1735-1740.
Stallings, Raymond L. (1994), "Distribution of Trinucleotide Microsatellites in Different Categories of Mammalian Genomic . . . " Genomics, 21:116-121.
Barron, Lilias, H. et al. (1994), "A single allele from the polymorphic CCG rich sequence immediately 3' to the unstable CAG trinucleotide in the IT15 cDNA shows almost complete disequilibrium with Huntington's disease chromosomes in the Scottish population" Human Molecular Genetics vol. 3, No. 1 173-175.
Koide, R. et al. (1994), "Unstable expansion of CAG repeat in hereditary dentatorubral-pallidoluysian atrophy (DRPLA)", Nature Genetics, 6:9-13.
Litt, Michael, et al. (1993), "Shadow Bands Seen When Typing Polymorphic Dinucleotide Repeats: Some Causes and Cures", Biotechniques, vol. 15:280-284.
Snell, Russell, G., (1993), "Relationship between trinucleotide repeat expansion and phenotypic variation in Huntington's disease", Nature Genetics, 4:393-397.
Knight, S. J. L. et al., "Trinucleotide Repeat Amplification and Hypermethylation of a CpG Island in FRAXE Mental Retardation", Cell, 74:127-134.
Orr, Harry, T. et al., (1993), "Expansion of an unstable trinucleotide CAG repeat in spinocerebellar ataxia type 1", Nature Genetics, 4:221-226.
Riess, Olaf, et al., (1993), "Improved PCR conditions for the stretch of (CAG).sub.n repeats causing Huntington's disease", Human Molecular Genetics, vol. 2, No. 6, p. 637.
Goldberg, Paul Y. et al. (1993) "A PCR method for accurate assessment of trinucleotide repeat expansion in Huntington Disease", Human Molecular Genetics, vol. 2, No. 6, 635-636.
Valdes, John M. et al. (1993), "A simple non-radioactive method for diagnosis of Huntinton's disease", Human Molecular Genetics, vol. 2, No. 6, 633-634.
The Huntington's Disease Collaborative Research Group (1993), "A Novel Gene Containing a Trinucleotide Repeat That Is Expanded and Unstable on Huntington's Disease Chromsomes" Cell 72:971-983.
Hauge, X. Y. et al. (1993), "A study of the orgin of `shadow bands` seen when typing dinucleotide repeat polymorphisms by the PCR" Human Molecular Genetics, vol. 2, No. 4, 411-415.
Rees, William, A. et al. (1993), "Betaine Can Eliminate the Base Pair Composition Dependence of DNA Melting", Biochemistry, 32:137-144.
Aslanidis, Charalampos, et al. (1992), "Cloning of the essential myotonic dystrophy region and mapping of the putative defect", Nature, 355:548-551.
Buxton, Jessica, et al. (1992), "Detection of an unstable fragment of DNA specific to individuals with myotonic dystrophy", Nature, 355:547-548.
Harley, Helen, G. (1992), "Expansion of an unstable DNA region and pheno-typic variation in myotionic dystrophy", Nature, 355:545-546.
Fu, Y. H. et al. (1992), "An Unstable Triplet Repeat in a Gene Related to Myotonic Muscular Dystrophy", Science, 255:1256-1258.
Andrew, Susan E. (1993), "The relationship between trinucleotide (CAG) repeat length and clinical features Huntington disease", Nature Genetics, 4:398-403.
La Spada, Albert R., et al. (1991), "Androgen receptor gene mutations in X-linked spinal and bulbar muscular atrophy", Nature, 352:77-79.
Kremer, E. J., et al. (1991), "Mapping of DNA Instability at the Fragile X to a Trinucleotide Repeat Sequence p(CCG).sub.n ", Science, 252:1711-1714.
Verkerk, Annemieke et al. (1991), "Identification of a Gene (FMR-1) Containing a CGG Repeat Coincident with a Breakpoint Cluster Region Exhibiting Length Variation in Fragile X Syndrome", Cell 65:905-914.
Yu, S. M. et al. (1991), "Fragile X Genotype Characterized by an Unstable Region of DNA", Science, 252:1179-1181.
Miller, S. A. (1988), "A simple salting out procedure for extracting DNA from human nucleated cells", vol. 16, No. 3, p. 1215.
Hippel, Peter H. et al. (1972), "DNA-Protein Interactions", Institute of Molecular Biology and Departments of Chemistry and Biology, U of Oregon, Eugene, pp. 231-300.
Melchior, William, B. et al. (1973), "Alteration of the Relative Stability of dA-dT and dG-dC Base Pairs in DNA", Proc.Nat. Acad. Sci. , vol. 70, No. 2, pp. 298-302.
Genzyme Corporation
Prats Francisco C.
Salcedo F. Brad
Wityshyn Michael G.
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