Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-14
1997-08-05
Arthur, Lisa B.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 9121, 935 77, 935 78, C12Q 168, C12P 1934
Patent
active
056541422
ABSTRACT:
The invention is an improved method for the amplification of nucleic acid, wherein ribonucleotides that weaken normal base pairing are introduced during amplification. Preferably, the ribonucleotides are inosine-triphosphate nucleotides which partly substitute guanine-triphosphate nucleotides normally present in the amplification reaction mixture. These ribonucleotides weaken normal base pairing and prevent the formation of secondary structures in the amplificate, thereby increasing the efficiency of amplification. Also, improved sensitivity results during detection of the amplified nucleic acid when the detection method comprises the hybridization of the amplified nucleic acid to a complementary sequence.
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Adriaanse Henriette Maria Aleida
Kievits Tim
Lens Peter Franklin
Akzo Nobel N.V.
Arthur Lisa B.
Blackstone William M.
Gormley Mary E.
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