Method for non-radioactive gel shift assays

Details Method for non-radioactive gel shift assays Method for non-radioactive gel shift assays

1997-07-24

1999-05-04

Marschel, Ardin H.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

436501, 935 77, 935 78, C12Q 168

Patent

active

059003588

ABSTRACT:
A method for a non-radioactive electrophoretic mobility shift assay performed with non-radioactive labeled dsDNA, non-radioactive labeled ssDNA or non-radioactive labeled RNA probes interacting with a nucleic acid binding protein in forming a complex, electrophoresing the mixture containing the complex, transferring the complex to a membrane, and detecting the complex transferred to the membrane by detecting the non-radioactive label in the complex.

REFERENCES:
patent: 4888274 (1989-12-01), Radding et al.
patent: 5273881 (1993-12-01), Sena et al.
Matthews et al. (1988) Analytized Biochemisty, vol. 169, pp. 1-25.
Suske et al., "Non-radioactive Method to Visualize Specific DNA-Protien Interactions in the Band Shift Assay", Nucleic Acids Research, vol. 17, No. 11, p. 4405, 1989.

Affiliated with

Also associated with

Rating

Method for non-radioactive gel shift assays does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for non-radioactive gel shift assays, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for non-radioactive gel shift assays will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1869394