Method for non-invasively determining the relative levels of...

Surgery – Means for introducing or removing material from body for... – Infrared – visible light – ultraviolet – x-ray or electrical...

Reexamination Certificate

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C600S345000

Reexamination Certificate

active

07555337

ABSTRACT:
The method for non-invasively determining the relative levels of two substances present in a biological system includes the steps of extracting by reverse iontophoresis charged and uncharged substances from said biological system, and collecting said substances, analysing the collected amount of a first extracted substance and a second extracted substance and determining the extraction ratio of the first substance to the second substance to determine their relative levels in the biological system. When the first and second substances are both analytes susceptible to changes in their concentration in the biological system, this method provides information about the relative concentrations of the two substances in the biological system. When the first substance is an analyte susceptible to changes in its concentration in the biological system and the second substance has a substantially constant concentration in the biological system, the second subtance acts as an internal standard and the extraction ratio becomes a direct measurement of the physiological level of the first substance in the biological system. A iontophoretic sampling device for monitoring the relative levels of two substances present in a biological system is also disclosed.

REFERENCES:
patent: 5279543 (1994-01-01), Cullander et al.
patent: 5730714 (1998-03-01), Glikfeld et al.
patent: 6059736 (2000-05-01), Tapper
patent: 6144869 (2000-11-01), Berner et al.
patent: 96/00110 (1996-01-01), None
International Search Report for PCT/EP02/06637; ISA/EPO; Mailed: Oct. 14, 2002.

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