Method for multiple analyte detection

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals

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436546, 436 89, 436164, 436807, 435 6, 435 71, 4352872, 4352885, 435973, G01N 35343, G01N 35533, G01N 3301, G01N 2700, C12O 168

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061241385

ABSTRACT:
The invention is directed to a method and device for simultaneously testing a sample for the presence, absence, and/or amounts of one or more a plurality of selected analytes. The invention includes, in one aspect, a device for detecting or quantitating a plurality of different analytes in a liquid sample. The device includes a substrate which defines a sample-distribution network having (i) a sample inlet, (ii) one or more detection chambers, and (iii) channel means providing a dead-end fluid connection between each of the chambers and the inlet. Each chamber may include an analyte-specific reagent effective to react with a selected analyte that may be present in the sample, and detection means for detecting the signal. Also disclosed are methods utilizing the device.

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