Method for more efficient electroporation

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S461000, C435S488000

Reexamination Certificate

active

06869797

ABSTRACT:
This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.

REFERENCES:
patent: 5186800 (1993-02-01), Dower
patent: 5712135 (1998-01-01), D'Halluin et al.
patent: 5879891 (1999-03-01), Thompson
patent: 6040184 (2000-03-01), Greener et al.
patent: 6338965 (2002-01-01), Greener et al.
patent: 6586249 (2003-07-01), Greener et al.
Andreason et al., 1988, “Introduction and Expression of DNA Molecules in Eukaryotic Cells by Electroporation,” BioTechniques, 6(7): 650-660.
Andreason et al., 1989, “Optimization of Electroporation for Transfection of Mammalian Cell Lines,” Analytical Biochemistry, 180: 269-275.
Antonov et al., 1993, “Heat Shock and Osmotically Dependent steps by DNA uptake afterEscherichia colielectroporation.”Biochemica et Biophysica Acta, 1216(2): 286-288.
Dower et al., 1988, “High Efficiency Transformation ofE. coliby High Voltage Electroporation,” Nucleic Acids Research, 16(13):6127-6145.
Dunny et al., 1991, “Improved Electroporation and Cloning Vector System for Gram-Positive Bacteria.” Applied and Environmental Microbiology, 57(4): 1194-1201.
Knutson et al., 1987, “Electroporation: Parameters Affecting Transfer of DNA into Mammalian Cells,” Analytical Biochemistry, 164: 44-52.
Neumann et al., 1982, “Gene Transfer into Mouse Lyoma Cells by Electroporation in High Electric Fields,” The EMBO Journal, 1(7): 841-845.
Sambrook et al., 1989, “Transformation ofE. coliby High-voltage Electroporation (Electrotransformation),” fromMolecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor Lab Press, p. 175.
Sambrook et al., 1989, “DNA Transfection by Electroporation,” fromMolecular Cloning, A Laboratory Manual, Second Edition, Cold Spring Harbor Lab Press, pp. 16.54-16.55.
Schnabel, 1998, “Protein-Protein Interactions Between Keratin Polypeptides Expressed in the Yeast Two-Hybrid System,” Biochemica et Biophysica Acta, 1403(2): 158-168.
Taketo, 1988, “DNA Transfection ofEscherichia coliby Electroporation,” Biochimica et Biophysica Acta, 949:318-324.
Thompson et al., 1998, “An Improved Protocol for the Preparation of Yeast Cells for Transformation by Electroporation,” Yeast, 14:565-571.
Wei, 1995, “An Improved Method for the Transformation ofLactobacillusStrains Using Electroporation,” Journal of Microbiological Methods, 21(1): 97-109.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for more efficient electroporation does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for more efficient electroporation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for more efficient electroporation will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3443316

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.