Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...
Patent
1999-02-22
2000-03-21
Ketter, James
Chemistry: molecular biology and microbiology
Process of mutation, cell fusion, or genetic modification
Introduction of a polynucleotide molecule into or...
4352523, 43525233, 435470, 435440, 435488, 435325, 435410, 43525421, C12N 1564
Patent
active
060401842
ABSTRACT:
This invention provides improved electroporation methods for transferring nucleic acids of interest into host cells, wherein the host cells are (1) suspended in a substantially non-ionic solution comprising at least one sugar or sugar derivative, (2) mixed with the nucleic acids of interest, and (3) electrically treated. Also, this invention provides for kits used in the method for transferring nucleic acids into host cells.
REFERENCES:
patent: 5186800 (1993-02-01), Dower
patent: 5712135 (1998-01-01), D'Halluin et al.
patent: 5879891 (1999-03-01), Thompson
Andreason et al., BioTechniques, 6:650-660 (1988).
Andreason et al., Analytical Biochemistry, 180:269-275 (1989).
Dower et al., Nucleic Acids Research, 16:6127-6145 (1988).
Knutson et al., Analytical Biochemistry, 164:44-52 (1987).
Neumann et al., The EMBO Journal, 1:841-845 (1982).
Sambrook et al., Molecular Cloning: A Laboratory Manual, 1:1.75, 2nd ed., Cold Spring Harbor Laboratory Press (1989).
Sambrook et al., Molecular Cloning: A Laboratory Manual, 316.54-16.55, 2nd ed., Cold Spring Harbor Laboratory Press (1989).
Taketo, Biochimica et Biophysica Acta, 949:318-324 (1988).
Greener Alan L.
Jerpseth Bruce D.
Ketter James
Stratagene
LandOfFree
Method for more efficient electroporation does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for more efficient electroporation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for more efficient electroporation will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-729789