Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-04-18
1998-11-24
Myers, Carla J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 435 63, 435 64, 536 2431, 536 2433, 935 8, 935 16, 935 17, 935 78, C12Q 168, C07H 2104, C12P 1934
Patent
active
058404945
ABSTRACT:
This invention provides a method of determining the stage of prostate cancer in a human subject using a sample of peripheral blood from a human subject. The invention uses reverse transcriptase-PCR to detect the mRNA encoding prostate specific antigen (PSA) in circulating cells expressing PSA. The invention further uses digoxigenin enhancement of the resultant cDNA signal. The method has an overall sensitivity capable of detecting one PSA expressing cell per 100,000 cells. The method produced no false positives and had a superior detection selectivity for patients with nonlocalized prostate cancer.
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Buttyan Ralph
Katz Aaron E.
Olsson Carl A.
Raffo Anthony
Myers Carla J.
The Trustees of Columbia University in the City of New York
White John P.
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