Method for modifying gene expression of a phytopathogenic...

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide confers pathogen or pest resistance

Reexamination Certificate

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C800S286000, C800S285000

Reexamination Certificate

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07741531

ABSTRACT:
The present invention relates to a system for controlling the development of fungi during a phytopathogenic attack which enables the plant to express a construct for inhibiting the expression of a gene essential to the development or to the pathogenicity of the fungus. The technology used is based on the mechanism of RNA interference. The present invention provides methods for producing plants resistant to a phytopathogenic fungus, plant cells and plants resistant to a phytopathogenic fungus, and methods of identifying a gene essential to the development or to the pathogenicity of a phytopathogenic fungus.

REFERENCES:
patent: 6506559 (2003-01-01), Fire et al.
patent: WO 99/32619 (1999-07-01), None
patent: WO 00/26346 (2000-05-01), None
patent: WO 01/96584 (2001-12-01), None
Kadotani et al. Sep. 2003, MPMI 16:769-776.
Kusaba et al. Jun. 2003, The Plant Cell 15:1455-1467.
Waterhouse et al., Nov. 1998, “Virus Resistance and Gene Silencing in Plants can be Induced by Simultaneous Expression of Sense and Antisense RNA.” Proceedings of the National Academy of Sciences of USA. (95): 13959-13964.
Tang, Guiliang, Brenda J. Reinhart, David P. Bartel and Philip D. Zamore, 2003, “A Biochemical Framework for RNA Silencing in Plants.” Genes & Development. (17, No. 1): 49-63.
Kadotani, Naoki, Hitoshi Nakayashiki, Yukio Tosa and Shigeyuki Mayama, Mar. 24, 2003, “RNA Silencing in the Phytopathic Fungus Magnaporthe oryzae.” Molecular Plant-Microbe Interactcions: MPMI. (16): 769-776.

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