Method for modifying chromosomes

Details Method for modifying chromosomes Method for modifying chromosomes

2003-09-11

2008-11-25

Kaushal, Sumesh (Department: 1633)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

C435S320100, C435S471000, C435S473000, C435S476000, C435S483000, C435S091200

Reexamination Certificate

active

07456020

ABSTRACT:
The present invention provides a simple method for splitting and loss of a chromosome in yeast. The method for modifying a chromosome in yeast includes preparing a linear chromosome splitting vector (1) having a target sequence (a), a marker gene sequence and (C4A2)nsequence in this order; preparing a linear chromosome splitting vector (2) having a target sequence (b), a centromere sequence of a yeast chromosome and (C4A2)nsequence in this order; and introducing the chromosome splitting vectors (1) and (2) into yeast. Herein, n is each independently an integer of 6 to 10. Although this chromosome splitting vector has a repetitive sequence of 5′-CCCCAA-3′, it can be amplified specifically with PCR, so that a chromosome splitting vector can be prepared significantly simply and easily, compared with the conventional DNA splitting method. It seems that a yeast telomere sequence is bound to the (C4A2)nsequence of the split chromosome generated by splitting with the chromosome splitting vector, and therefore the fragment can function as an intact chromosome.

REFERENCES:
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