Multicellular living organisms and unmodified parts thereof and – Nonhuman animal – Transgenic nonhuman animal
Reexamination Certificate
2011-01-11
2011-01-11
Ton, Thaian N (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Nonhuman animal
Transgenic nonhuman animal
C800S014000, C800S021000, C800S025000
Reexamination Certificate
active
07868223
ABSTRACT:
The present invention relates to a method for producing a modified foreign chromosome(s) or a fragment(s) thereof, which comprises the steps of: (a) preparing a microcell comprising a foreign chromosome(s) or a fragment(s) thereof, and transferring said foreign chromosome(s) or a fragment(s) into a cell with high homologous recombination efficiency through its fusion with said microcell; (b) in said cell with high homologous recombination efficiency, inserting a targeting vector by homologous recombination into a desired site of said foreign chromosome(s) or a fragment(s) thereof, and/or a desired site of a chromosome(s) derived from said cell with high homologous recombination efficiency, thereby marking said desired site; and (c) in said cell with high homologous recombination efficiency, causing deletion and/or translocation to occur at the marked site of said foreign chromosome(s) or a fragment(s) thereof.
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Weichh
Hanaoka Kazunori
Ishida Isao
Kuroiwa Yoshimi
Oshimura Mitsuo
Tomizuka Kazuma
Bent Stephen A.
Foley & Lardner LLP
Kyowa Hakko Kirin Co., Ltd.
Ton Thaian N
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