Liquid purification or separation – Processes – Using magnetic force
Patent
1997-07-29
2000-03-07
Savage, Matthew O.
Liquid purification or separation
Processes
Using magnetic force
436526, B01D 3506
Patent
active
060335742
ABSTRACT:
An apparatus and method for carrying out the affinity separation of a target substance from a liquid test medium by mixing magnetic particles having surface immobilized ligand or receptor within the test medium to promote an affinity binding reaction between the ligand and the target substance. The test medium with the magnetic particles in a suitable container is removably mounted in an apparatus that creates a magnetic field gradient in the test medium. This magnetic gradient is used to induce the magnetic particles to move, thereby effecting mixing. The mixing is achieved either by movement of a magnet relative to a stationary container or movement of the container relative to a stationary magnet. In either case, the magnetic particles experience a continuous angular position change with the magnet. Concurrently with the relative angular movement between the magnet and the magnetic particles, the magnet is also moved along the length of the container causing the magnetic field gradient to sweep the entire length of the container. After the desired time, sufficient for the affinity reaction to occur, movement of the magnetic gradient is ended, whereby the magnetic particles are immobilized on the inside wall of the container nearest to the magnetic source. The remaining test medium is removed while the magnetic particles are retained on the wall of the container. The test medium or the particles may then be subjected to further processing.
REFERENCES:
patent: 4230685 (1980-10-01), Senyei et al.
patent: 4390283 (1983-06-01), Meyer
patent: 4554088 (1985-11-01), Whitehead et al.
patent: 4628037 (1986-12-01), Chagnon et al.
patent: 4752138 (1988-06-01), Rufer
patent: 4895650 (1990-01-01), Wang
patent: 4910148 (1990-03-01), Sorenson et al.
patent: 5043063 (1991-08-01), Latimer
patent: 5238812 (1993-08-01), Coulter et al.
patent: 5336760 (1994-08-01), Hardwick et al.
patent: 5628407 (1997-05-01), Gilbert et al.
"Magnetized Monosized Polymer Particles for Fast and Specific Fractionation of Human Mononuclear Cells" by T. Lea, F. Vartdal, C. Davies & J. Ugelstad, Scandinavian Journal of Immunology, vol. 22, pp. 207-216, Mar. 8, 1985.
"Depletion of T Lymphocytes from Human Bone Marrow" by F. Vartdal, G. Kvalheim, T. Lea, V. Dosnes, G. Gaudernack, J. Ugelstad, D. Albrechtsen, Transplantation, vol. 43, No. 3, pp. 366-371, 1987.
"Removal of Neuroblastoma Cells from Bone Marrow with Monoclonal Antibodies Conjugated to Magnetic Microspheres" J. Treleaven, J. Ugelstad, T. Philips, F. Gibson, A. Rembaum, G. Caine, J. Kemshead, The Lancet, Jan. 14, 1984, pp. 70-73.
"Application of Magnetic Beads in Bioassays" b. Haukanes and C. Kvam, Bio/Technology, vol. 11, pp. 60-62, Jan. 1993.
RIA Kits Catalog, undated, Separator Units, Catalog Nos. 8-4001K, 8-4101S, 8-4102X, 8-4106S, 8-4109S, and 8-4104S.
Dynal Catalog, undated pp. 30-32, Magnetic Equipment, Mixing Equipment, Dynal Sample Mixer.
Sigris Research, Inc. Flyer--MixSep Biomagnetic Separations in Molecular Biology, 1996.
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