Chemistry: molecular biology and microbiology – Plant cell or cell line – per se ; composition thereof;...
Reexamination Certificate
2010-11-22
2011-10-04
McCormick Ewoldt, Susan (Department: 1661)
Chemistry: molecular biology and microbiology
Plant cell or cell line, per se ; composition thereof;...
C435S420000, C435S430000, C435S430100
Reexamination Certificate
active
08030073
ABSTRACT:
The present invention provides a method of micropropagating a monocotyledonous plant comprising: (a) cultivating an explant of tissue from a monocotyledonous plant shoot tip on a primary medium, wherein the explant has been pretreated with a cold temperature and the primary medium comprises auxin or auxin and cytokinin, to produce a totipotent embryogenic cell culture; (b) treating the totipotent embryonic cell culture with a cold temperature; (c) maintaining the totipotent embryogenic cell culture by cultivation on a secondary medium, whereby a totipotent embryogenic cell culture of a monocotyledonous plant is produced and maintained; and (d) transferring the embryogenic cell culture of step (c) to a tertiary medium to continue multiplication and to produce a plantlet with roots and shoots, thereby micropropagating a monocotyledonous plant. The micropropagation techniques described herein provide plants for such purposes as development of elite plant lines, phytoremediation and biomass production.
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Czako Mihaly
Márton László
Ewoldt Susan McCormick
Myers Bigel Sibley & Sajovec P.A.
The University of South Carolina
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