Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1979-11-05
1982-12-28
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435172, 435317, 536 27, C12P 2100, C12N 1500, C12N 100
Patent
active
043662463
ABSTRACT:
The Specification discloses:
1. Recombinant microbial cloning vehicles comprising heterologous DNA coding for the expression of mammalian hormone (e.g., somatostatin) and other polypeptides, including plasmids suited for the transformation of bacterial hosts. The latter incorporate a regulon homologous to the host in its untransformed state, in reading phase with the structural gene for the heterologous DNA;
2. Cloning vehicles coding for the microbial expression of a protein variously comprising (a) a polypeptide hapten and additional protein sufficient in size to confer immunogenicity on the product of expression, which may find use in raising antibodies to the hapten for assay use or in the manufacture of vaccines; and (b) a desired polypeptide product and additional protein from which the desired product may be cleaved; and
3. Methods of preparing synthetic structural genes coding for the expression of mammalian polypeptides in microbial cloning systems.
REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
Itakura et al., Science, vol. 198, pp. 1056-1063, Dec. 9, 1977.
O'Farrell et al., J. Bacteriology, vol. 134, pp. 645-654 (May 1978).
Casadaban, J. Mol. Biol., vol. 104, pp. 541, 557 (1976).
Polisky et al., Proc. Natl. Acad. Sci., vol. 73, No. 14, pp. 3900-3904 (Nov. 1976).
Higuchi et al., Proc. Natl. Acad. Sci., vol. 73, No. 9, pp. 3146-3150 (Sep. 1976).
Ullrich et al., Science, vol. 196, pp. 1313-1319 (Jun. 17, 1977).
Genentech Inc.
Kiley Thomas D.
Tanenholtz Alvin E.
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