Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1990-07-02
1992-03-03
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
435170, 435182, 43525233, 435849, C12N 1110, C12N 1104, C12N 120, C12P 104
Patent
active
050932535
ABSTRACT:
Microbial cells are immobilized by entrapment in gellan gum, also known as deacetylated heteropolysaccharide S-60. Entrapment can be carried out by forming a mixture of a paste of microbial cells and an aqueous solution of gellan gum and adding the mixture drop-wise to an aqueous solution of cations to produce beads of hardened gellan gum entrapping the microbial cells. The microbial cells preferably contain aspartase activity and can be E. coli ATCC 11303, and the cations are preferably magnesium ions. In an alternative embodiment, the mixture of microbial cell paste and aqueous gellan gum solution is admixed with a porous cationic exchange resin which is preferably in magnesium ion form and the microbial cells are entrapped in hardened gellan gum in and on the resin.
REFERENCES:
patent: 4352883 (1982-10-01), Lim
patent: 4452892 (1984-06-01), Rosereer
patent: 4526867 (1985-07-01), Chibata et al.
patent: 4701326 (1987-10-01), Nelson et al.
Monsanto Company
Naff David M.
Passley Paul L.
Williams, Jr. James W.
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