Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide
Patent
1985-09-03
1990-10-30
Weimar, Elizabeth C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using a micro-organism to make a protein or polypeptide
435259, 435261, 435800, 435839, 435849, 435875, C12P 2100, C12N 106, C12N 102
Patent
active
049668447
ABSTRACT:
In processes for recovery of biologically active polypeptides from fermentation cultures of recombinant host organisms, cell death is frequently a prerequisite for isolation processing of the recombinant product outside the fermentation vessel. Disclosed are improved methods for effecting efficient host cell death inside the fermentation vessel through uniformly contacting host cells in culture with microbicidal concentrations of benzyl alcohol. Illustratively, E. coli, B. subtilis, and P. aeruginosa cultures are advantageously treated with from 0.5 to 10.0% (v/v) of benzyl alcohol in the absence of pH or temperature changes within the fermentor.
REFERENCES:
patent: 4560491 (1985-12-01), Sherman
Akers, Considerations in Selecting Antimicrobial Preservative Agents for Parenteral Product Development, Pharmaceutical Technology, 36-46, May 1984.
Merck Index, 10th Edition (1983).
Wang and Kowal, Review of Excipients and pH's for Parenteral Products Used in the United States, J. Parenteral Drug Association, vol. 34, No. 6, pp. 452-462 (Nov.-Dec. 1980).
Wetzel, et al., Production of Biologically Active N.sup..alpha. -Desacetylthymosin .alpha..sub.1 in E. coli through Expression of Chemically Synthesized Gene, Biochemistry, 19, 6096-6104 (1980).
Fenton Dennis M.
Tallen Michael J.
Amgen
Patterson Jr. Charles L.
Weimar Elizabeth C.
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