Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2005-09-13
2005-09-13
Wax, Robert (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S252300, C435S128000, C435S155000, C435S189000
Reexamination Certificate
active
06943006
ABSTRACT:
A method for selective cleavage of C—N bonds genes that encode for at least one enzyme suitable for conversion of carbazole to 2-aminobiphenyl-2,3-diol are combined with a gene encoding an amidase suitable for selectively cleaving a C—N bond in 2-aminobiphenyl-2,3-diol, forming an operon that encodes for cleavage of both C—N bonds of said carbazole. The operon is inserted into a host culture which, in turn, is contacted with the carbazole, resulting in selective cleavage of both C—N bonds of the carbazole. Also disclosed is a new microorganism that expresses a carbazole degradation trait constitutively and a method for degrading carbazole employing this microorganism.
REFERENCES:
patent: 3276840 (1966-10-01), Gonzalo
patent: 5856167 (1999-01-01), Outtrup
patent: 5888797 (1999-03-01), Outtrup
patent: 5928929 (1999-07-01), Outtrup et al.
Sato et al., “Cloning of genes involved in carbazole degradation of Pseudomonas sp. strain CA10: nucleotide sequences of genes and characterization of meta-cleavage enzymes and hydrolase,” J Bacteriology 179(15):4841-4849, 1997.
Enzyme Nomenclature (on-line), record for subtilisin (EC 3.4.21.62), published by Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB), http://www.chem.qmul.ac.uk/iubmd/enzyme, printed Jun. 24, 2004.
Kilbane et al., “Isolation and characterization of Sphingomonas sp. GTIN11 capable of carbazole metabolism in petroleum,” Biochem Biophys Res Comm 297(2):242-248, available on line Sep. 4, 2002.
Ouchiyama, Naoki et al., “Cloning and Nucleotide Sequence of Carbazole Catabolic Genes fromPseudomonas stutzeristrain OM1, Isolated from Activated Sludge”,J. Gen. Appl. Microbiol., 44, 57-63 (1998).
Shotbolt-Brown, J. et al., “Isolation and Description of Carbazole-Degrading Bacteria”,Can. J. Microbiol., 42 79-82 (1996).
Schneider, J. et al., “Biodegradation of Carbazole byRalstoniasp. RJGII. 123 isolated From a Hydrocarbon Contaminated Soil”,Can. J. Microbiol., 46 269-277 (2000).
Ouchlyama, N. et al., “Biodegradation of Carbazole byPseudomonasspp. CA06 and CA10”,Biosci. Biotech. Biochem., 57 (3), 455-460 (1993).
Hisatsuka, K. et al., “Microbial Transformation of Carbazole to Anthranilic Acid byPseudomones stutzen”, Biosci. Biotech. Biochem., 58 (1), 213-214 (1994).
Shao, Z. Q. et al., “Cloning and Expression of the s-Triazine Hydrolase Gene (trzA) fromRhodococcus corallinusand Development ofRhodococcusRecombinant Strains Capable of Dealkylating and Dechlorinating the Herbicide Atrazine”,Journal of Bacteriology, vol. 177, No. 20, pp. 5748-5755 (Oct. 1995).
Trott, S. et al., “Cloning and Heterologous Expression of an Enantioselective Amidase fromRhodococcus erythropolisStrain MP50”,Applied and Environmental Microbiology, vol. 68, No. 7, pp. 3279-3286 (Jul. 2002).
Grosser, R. J. et al., “Indigenous and Enhanced Mineralization of Pyrene, Benzo[a]pyrene, and Carbazole in Soils”,Applied and Environmental Microbiology, vol. 57, No. 12, pp. 3462-3469 (Dec. 1991).
Lasa, I. et al., “Development ofThermus-EscherichiaShuttle Vectors and Their Use for Expression of theClostridium thermocellum ceIAGene inThermus thermophilus”, Journal of Bacteriology, vol. 174, No. 20, pp. 6424-6431 (Oct. 1992).
Aronstein, B. N. et al., “Biological and Integrated Chemical-Biological Treatment of PCB Congeners in Soil/Sediment-Containing Systems”,J. Chem. Tech. Biotechnol., 63, 321-326 (1995).
Kilbane II, J. J. et al., “Selective Removal of Nitrogen from Quinoline and Petroleum byPseudomones ayucideIGTN9m”,Applied Environmental Microbiology, vol. 66, No. 2, pp. 688-693 (Feb. 2000).
Nojiri, H. et al., “Genetic Characterization and Evolutionary Implications of a car Gene Cluster in the Carbazole DegraderPseudomonessp. Strain CA10”,Journal of Bacteriology, vol. 183, No. 12, pp. 3663-3679 (Jun. 2001).
Kayser, K. J. et al., “Utilization of Organosulphur Compounds by Axenic and Mixed Cultures ofRhodococcus rhodochrousIGTS8”Journal of General Microbiology, 139, 3123-3129 (1993).
Watson, G. K. et al., “Microbial Metabolism of the Pyrindine Ring—Metabolic Pathways of Pyridine Biodegradation by Soil Bacteria”,Biochem. J., 146, 157-172 (1975).
Furukawa, K. et al., “Cloning of a Gene Cluster Encoding Biphenyl and Chlorobiphenyl Degradation inPseudomonas pseudoalcaligenes”, Journal of Bacteriology, vol. 166, No. 2, pp. 392-398 (May 1986).
Reese, M. G. et al., “Large Scale Sequencing Specific Neural Networks for Promoter and Splica Site Recognition”,Pacific Symposium on Biocomputing, pp. 737-738, (Jan. 3-6, 1996).
Shepherd, J. M. et al., “Novel Carbazole Degradation Genes ofSphingomonasCB3: Sequence Analysis, Transcription, and Molecular Ecology”Biochemical and Biophysical Research Communications, 247, 129-135 (1998).
Nojiri, H. et al., “Organization and Transcriptional Characterization of Catechol Degradation Genes Involved in Carbazole Degradation byPseudomonas resinovoransStrain CA10”Biosci. Biotechnol. Biochem., 66 (4), 897-901 (2002).
Kirimura, K. et al., “Selective and Continuous Degradation of Carbazole Contained in Petroleum Oil by Resting Cells ofSphingomonassp CDH-7”,Biosci. Biotechnol. Biochem., 63 (9), 1563-1568 (1999).
Loh, K. et al., “Kinetics of Carbazole Degradation byPseudomonas putidein Presence of Sodium Salicylate”,Wat. Res., vol. 34, No. 17, pp. 4131-4138 (2000).
Ellis, L. B. M. et al., “The University of Minnesota Biocatalysis/Biodegradation Degradation: Emphasizing Enzymes”,Nucleic Acids Research, vol. 29, No. 1, pp. 340-343, (2001).
Maidak, B. L. et al., “The RDP-II (Ribosomal Database Project)”,Nucleic Acids Research, vol. 29, No. 1, pp. 173-174 (2001).
Habe, H. et al., “Sphingomonassp. Strain KA1, Carrying a Carbazole Dioxygenase Gene Homologue, Degrades Chlorinated Dibenzo-p-dioxins in Soil”,FEMS Microbiology Letters211 43-49 (2002).
Sato, S. Et al., “Identification and Characterization of Genes Encoding Carbazole 1,9a-Dioxygenase inPseudomonassp. Strain CA10”,Journal of Bacteriology, vol. 179, No. 15, pp. 4850-4858, (Aug. 1997).
Kayser Kevin J.
Kilbane II John J.
Alps Electric Co. ,Ltd.
Kosson Rosanne
Wax Robert
LandOfFree
Method for metabolizing carbazole in petroleum does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for metabolizing carbazole in petroleum, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for metabolizing carbazole in petroleum will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3420503