Method for measuring the free fraction of ligands in biological

Details Method for measuring the free fraction of ligands in biological Method for measuring the free fraction of ligands in biological

1993-02-11

1994-01-11

Nucker, Christine M.

Chemistry: analytical and immunological testing

Thyroid hormone tests

436501, 436504, 435 71, 435 793, G01N 3353, G01N 33566, G01N 33567, C12Q 100

Patent

active

052780805

ABSTRACT:
A one-step assay for the free portion of a ligand in a biological sample involves incubating a mixture of the sample with a labelled antibody for the ligand and a ligand analogue which competes with the ligand for binding to the antibody. The assay is characterized by choosing a ligand analogue which has a lower affinity for the antibody than does the ligand. An insolubilised ligand analogue preferably has a binding affinity for the antibody from 0.01% to 10% of that of the ligand. Ligand/ligand analogue pairs exemplified are T4/T3 (Thyroxine/Tri- iodothyronine); Testosterone/etiocholanol; and T3/T2.

REFERENCES:
patent: 4292296 (1981-09-01), Parsons, Jr.
patent: 4366143 (1982-12-01), Midgley et al.

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