Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1998-08-18
2000-10-10
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 23, 435184, 435962, C12Q 134
Patent
active
061300551
ABSTRACT:
A method for measuring the concentration or the activity of UTI quickly and easily at high sensitivity. A urine sample, a buffer solution, a trypsin solution and a substrate solution are mixed and the trypsin activity is then measured. Thus, the UTI concentration in the urine sample is determined. In this case, a substrate solution having only L-BAPNA is used as the substrate, and the surfactant is mixed in at least one selected from the buffer solution and the enzyme solution. The mixing ratio of the surfactant is about 1 wt. % in the entire enzyme reaction solution. Examples of the surfactant include polyoxyethylene (40) octylphenylether, polyoxyethylene (10) octylphenylether, 3-[(3-cholamido propyl)dimethylammonio]-propanesulfonic acid, 3-[(3-cholamido propyl)dimethylammonio]-2-hydroxypropanesulfonic acid, and polyoxyethylene sorbitan monolaurate. As shown in FIG. 1, the sensitivity improves when using L-BAPNA.
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Fukunaga Satoshi
Nanbu Atsuko
Gitomer Ralph
Kyoto Dai-ichi Kagaku Co. Ltd.
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