Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1994-07-05
1997-06-03
Knode, Marian C.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435968, 436504, 436804, G01N 3360
Patent
active
056353596
ABSTRACT:
The activity of angiotensin converting enzyme (ACE) is measured in biological samples as body fluids. ACE-activity is estimated in minimally diluted specimens, using the natural substrate angiotensin I at close to physiological concentration. Femtomoles of generated angiotensin II are trapped by specific high affinity monoclonal antibodies and thus protected from degradation by angiotensinases during the incubation step. The same antibodies are subsequently used for quantitation by radioimmunoassay.
REFERENCES:
patent: 5407803 (1995-04-01), Brunner et al.
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Nussberger, J., et al., "True Versus Immunoreactive Angiotensin II in Human Plasma," Suppl. I. Hypertension 7:I-1 to I-7, 1985.
Nussberger, J., et al., "Selectivity of Angiotensin II Antisera," Journal of Immunological Methods 56: 85-96, 1983.
Poulsen and Jorgensen, "An Easy Radioimmunological Microassay of Renin Activity, Concentration and Substrate in Human and Animal Plasma and Tissues Based on Angiotensin I Trapping by Antibody," The Journal of Clinical Endocrinology and Metabolism 39:816-825, 1974.
Poulsen, Knud, "Simplied Method for Radioimmunossay of Enzyme Systems," The Journal of Laboratory and Clinical Medicine 78:309-315, 1971.
Nussberger et al., "Angiotensen II Measurement with High-Affinity Monoclonal Antibodies," Journal of Hypertension 6 Suppl. 4:S424-S425, 1988.
Nussberger et al., "Measurement of Converting Enzyme Activity by Antibody-Trapping of Generated Angiotensin II," Am. J. Hypertension 5:393-398, 1992.
Brunner Hans R.
Nussberger Jurg
B.M.R.A. Corporation B.V.
Knode Marian C.
Wortman Donna C.
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