Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1992-01-31
1995-04-18
Scheiner, Toni R.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435968, 436548, 436504, 436804, G01N 33573
Patent
active
054078032
ABSTRACT:
The activity of angiotensin converting enzyme (ACE) is measured in biological samples as body fluids. ACE-activity is estimated in minimally diluted specimens, using the natural substrate angiotensin I at close to physiological concentration. Femtomoles of generated angiotensin II are trapped by specific high affinity monoclonal antibodies and thus protected from degradation by angiotensinases during the incubation step. The same antibodies are subsequently used for quantitation by radioimmunoassay.
REFERENCES:
Nussberger et al., Am. J. Hypertension 5: 393-398, 1992.
Poulsen and Jorgensen, "An Easy Radioimmunological Microassay of Renin Activity, Concentration and Substrate in Human and Animal Plasma and Tissues Based on Angiotensin I Trapping by Antibody" The Journal of Clinical Endocrinology and Metabolism 39:816-825, 1974.
Knud Poulsen, "Simplified Method for Radioimmunoassy of Enzyme Systems" The Journal of Laboratory and Clinical Medicine 78:309-315, 1971.
Nussberger et al., "Angiotensin II Measurement with High-Affinity Monoclonal Antibodies" Journal of Hypertension 6 Suppl 4:S424-S425, 1988.
Brunner Hans R.
Nussberger Juerg
B.M.R.A. Corporation B.V.
Scheiner Toni R.
Wortman Donna C.
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