Method for measuring specific binding assays

Chemistry: analytical and immunological testing – Automated chemical analysis – Utilizing a centrifuge or compartmented rotor

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436164, 436165, 436172, 422 64, 422 67, 422 72, 422 8205, 422 8208, 422 8209, 356426, G01N 2117, G01N 2164

Patent

active

054496217

ABSTRACT:
An automated patient sample analysis instrument uses assay cartridges which are pre-loaded with all reactants needed to perform an assay. Photometric measurements are performed on the cartridges while spinning at variable speeds in a centrifuge. Both axial and transverse measurements can be made on the cartridges. Multiple optical channels can be read by single detector. The instrument is well suited for low volume and for use by unskilled individuals. The photometric measurements are made by dividing the width of a measurement window into n intervals, measuring an optical result in one of the n intervals in each of n rotations of the cartridge, repeating the measurement process x times, summing or averaging the x results for each of the n intervals and selecting y of the largest resultant averaged or summed measurements as representative of the maximum measurement for the window.

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