Method for measuring sample by enzyme electrodes

Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy

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2041531, G01N 2726

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active

054075459

ABSTRACT:
A buffer solution is charged in a reaction container so that enzyme electrodes can be immersed in the buffer solution. Current values between the enzyme electrodes, to which a voltage is applied, are sampled at predetermined-time intervals. A decreasing curve of current is predicted on the basis of the sampled current values. A sample is injected in said buffer solution at an early time before the current between enzyme electrodes become stable, whereby oxidation reaction of a specified component of the sample is caused. A current value I.sub.1 between enzyme electrodes is measured and simultaneously a current value T.sub.2 on the decreasing curve is obtained at a point that a predetermined time passes from the sample injection point to obtain a current increase from the sample injection on the basis of a difference of the current values I.sub.1 and I.sub.2. A quantity of the specified component of the sample is obtained on the basis of the current increase.

REFERENCES:
patent: 4767994 (1988-08-01), Hopkins et al.
patent: 5312528 (1994-05-01), Hoogendijk
patent: 5320939 (1994-06-01), Hashizume et al.
Patent Abstracts of Japan, vol. 007, No. 026 no month or year available.
Database WPI, Section Ch, Week 8302, Derwent Publications Ltd., London no month or year available.

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