Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving urea or urease
Patent
1978-12-06
1980-04-08
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving urea or urease
435 4, 435 18, 435 20, 435 25, 435 26, 435 27, 435 28, 435 29, 435 39, 435 34, 435807, C12Q 158
Patent
active
041973690
ABSTRACT:
A method and apparatus is provided for determining the concentration or quantity of enzyme molecules, microorganisms or substrate molecules by monitoring directly the concentration and/or the quantity of vaporous by-product produced from the selective reaction of the molecule catalyzed with a known amount of substrate or of an enzyme or a microorganism. The apparatus comprises a membrane permeable to the vaporous product or substrate, a known amount of microorganism or an enzyme adjacent to the membrane, means for introducing a liquid sample into contact with the microorganism or enzyme within a small volume adjacent the membrane and means for measuring the amount of vaporous by-product passing through the membrane. A mass spectrometer located adjacent the membrane surface is suitable for measuring the amount of vaporous product or substrate passing through the membrane. The process is conducted by first calibrating the apparatus with a known concentration of substrate or of enzyme, and thereafter contacting a sample containing an unknown concentration of enzyme or microorganism with the substrates, or substrate with the enzyme or microorganism.
REFERENCES:
patent: 3700562 (1972-10-01), Morgenstern et al.
patent: 3948731 (1976-04-01), Weaver
Massachusetts Institute of Technology
Warden Robert J.
LandOfFree
Method for measuring reactant concentrations and quantities does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for measuring reactant concentrations and quantities, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for measuring reactant concentrations and quantities will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-974235