Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving blood clotting factor
Patent
1994-08-04
1997-02-04
Gitomer, Ralph
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving blood clotting factor
435 23, 435 692, 436 69, C12Q 156, G01N 3386
Patent
active
055996833
ABSTRACT:
The present invention provides a method of measuring the activity of a substance to test how it inhibits or promotes the production of the blood coagulation factor XII in an active form (FXIIa). An activation reaction of the blood coagulation factor XII is initiated by adding a blood coagulation factor XII activator to an animal plasma in the presence of the tested substance. Then the reaction is stopped and the blood coagulation factor XII in an active form (FXIIa) produced in the above reaction is quantitatively determined. FXIIa plays a very important role in the initiation stage in the intrinsic blood clotting system, fibrinolysis system and plasma kallikrein-kinin system. Accordingly, the method of the present invention in which the activity of the tested substance having an inhibiting or promoting action on the production of FXIIa is measured is very highly useful in screening compounds or drugs related to blood clotting and fibrinolysis systems. It may also be used to screen compounds or drugs related to the plasma kallikrein-kinin system such as, for example, blood pressure controlling agents, antiinflammatory agents, analgesics, antiallergic agents, etc.
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Ishibashi Nobuyuki
Nishikawa Katsumi
Gitomer Ralph
Nippon Zoki Pharmaceutical Co. Ltd.
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