Method for measuring no synthase activity

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S004000, C435S007100

Reexamination Certificate

active

07045307

ABSTRACT:
A method for measuring the activity of NO-synthase, a corresponding method for identification of NO-synthase modulators, in particular for application in a HTS (High Throughput Screening) and use of the corresponding cation-exchange filter-plate membranes.

REFERENCES:
patent: 4058456 (1977-11-01), Head
patent: 5629322 (1997-05-01), Guthikonda et al.
patent: 5972975 (1999-10-01), Esser et al.
patent: 2003/0022914 (2003-01-01), Maul et al.
patent: 19902387 (2000-07-01), None
Dawson et al, A microtiter-plate assay of nitric oxide synthase activity. Molecular Biotechnology, 1999, vol. 12, pp. 275-279.
Gross, S., Microtiter plate assay for determining kinetics of nitric oxide synthesis. Methods in Enzymology, 1996, vol. 268, pp. 159-168.
McCarty, M., Purification and properties of desoxyribonuclease isolated from beef pancreas. Journal of General Physiology, 1946, pp. 123-139.
David S. Bredt, et al., “Isolation of Nitric Oxide Synthetase, a calmodulin-requiring Enzyme” Proc. National. Acad. Of Science, vol. 87, Jan. 1990, pp. 682-685.
David S. Bredt, et al., “Nitric oxide mediates glutamate-linked enhancement of cGMP levels in the cerebellum” Proc. National Acad. Of Science, vol. 86, Nov. 1989, pp. 9030-9033.
J. M. Cunningham, et al., “Interleukin-1β-Mediated Inhibition of Arginase in RINm5F Cells” Cytokine, vol. 9, No. 8, Aug. 1997, pp. 570-576.
Richard G. Knowles, et al., “Measurement of NOS Activity by Conversion of Radiolabeled Arginine to Citrulline Using Ion-Exchange Separation” Methods in Molecular Biology, vol. 100, Nitric Oxide Protocols, pp. 67-73.
“Blotting and Chromatography Papers” www.whatman.plc.uk/products/analytical/labfiltration/a—pd—labfil—002.html.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Method for measuring no synthase activity does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for measuring no synthase activity, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for measuring no synthase activity will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3623025

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.