Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Glycoproteins
Patent
1998-03-23
1999-08-03
Soderquist, Arlen
Chemistry: analytical and immunological testing
Peptide, protein or amino acid
Glycoproteins
436 86, 436 63, 436161, 436164, 436166, 436174, 436175, 436177, G01N 3348
Patent
active
059324813
ABSTRACT:
A method for the rapid estimation of hyperplastic and hypertrophic changes in animal airways is an assay which specifically measures acidic and neutral mucoproteins in a linear fashion from 0.5 to at least 10 .mu.g. The assay comprises exposure of a test animal to a suspected metaplastic inducer, removal of the lungs, homogenization in an appropriately buffered solution containing reducing agents and protease inhibitors; removal of particulate matter; and size-fractionation of the SDS treated soluble extract. The high molecular weight material is immobilized and stained for either acidic or neutral mucosubstances and the specific staining is quantitated. The changes observed are consistent with those seen in histological sections of the exposed tissues. The assay is useful in confirming the metaplastic potential of suspected compounds, in determining what neurohumoral mediator(s) are involved in mucus cell metaplasia in animal models for chronic obstructive pulmonary disease, and in identifying compounds which might ameliorate these effects.
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Boulet Louise
Fortin Rejean
Pon Douglas J.
van Staden Carlo J.
Giesser Joanne M.
Hand J. Mark
Merck Frosst Canada & Co.
Soderquist Arlen
Tribble Jack L.
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