Chemistry: analytical and immunological testing – Nuclear magnetic resonance – electron spin resonance or other...
Patent
1992-04-29
1994-08-16
Warden, Robert J.
Chemistry: analytical and immunological testing
Nuclear magnetic resonance, electron spin resonance or other...
424 9, 436174, 436177, G01N 3100, G01N 2400, G01N 33543
Patent
active
053386868
ABSTRACT:
A method for determining the mass isotope enrichment of a subunit from which a biopolymer is formed, and the rates of synthesis and decay of the biopolymer. The mass isotope enrichment of the subunit is determined by comparing the mass isotopomer distribution of the biopolymer after administration of a mass isotopically labeled subunit, with the expected frequencies of the different mass isotopomers produced from a given subunit mass isotope enrichment. To determine the synthesis rate of the biopolymer, the expected frequency of a selected biopolymer mass isotopomer, calculated from the subunit isotope enrichment, is compared with the actual frequency of that biopolymer mass isotopomer. To determine biopolymer decay, the decay rate of high mass isotopomers of the biopolymer is determined.
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Dehlinger Peter J.
Stratford Carol A.
Torres Ramon
Warden Robert J.
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