Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1994-08-22
1996-03-26
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 4, 435 14, 435 97, 435810, 436 94, 436 92, 536 2911, 536 111, 514 54, 514 23, 514 1, C12Q 148, C12Q 100, G01N 3300, A01N 4304
Patent
active
055019579
ABSTRACT:
A method of assaying for glycosyltransferase activity in a sample. In a first step, a sample is reacted with a first sugar donor and an acceptor substrate to produce a transferase product. The first sugar donor and acceptor substrate are selected such that the sugar from the first sugar donor is capable of being transferred to the acceptor substrate in the presence of the glycosyltransferase to be assayed. In a second step, the transferase product is reacted with a second sugar donor having a sugar which is labelled with a labelling agent and an enzyme which is capable of transferring the sugar from the second sugar donor to the transferase product to produce a labelled transferase product and which has a higher affinity for the glycosyltransferase product compared to the affinity of the glycosyltransferase for the acceptor substrate. The labelling agent activity of the labelled transferase product or unreacted second sugar donor is assayed to determine transferase activity in the sample. A kit for assaying for glycosyltransferase activity in a sample is also described.
REFERENCES:
patent: 4205129 (1980-05-01), Podolsky et al.
patent: 5109126 (1992-04-01), Agrawal et al.
patent: 5173407 (1992-12-01), Vemura et al.
patent: 5352670 (1994-10-01), Venot et al.
Verdon and Berger, Methods of Enzymatic Analysis, 3:374-381, 1986.
Rademacher, T. W. et al, Ann. Rev. Biochem. 57:785, 1988.
Yousefi, S. et al, J. Biol. Chem. 266:1772, 1991.
Dennis, J. W. et al, Science, vol. 236, pp. 582-585, 1987.
Warren L. et al. Biochem. Biophys. Acta. 516:97, 1978.
Piller, F. et al, J. Biol. Chem. 263:15146, 1988.
Higgins, L. A. J. Biol. Chem. 266:6280, 1991.
Brockhausen, I. et al Cancer Res. 51:1257, 1991.
Paulson, J. C. et al J. Biol. Chem. 264, pp. 10931-10934, 1989.
Wang, X et al Glycobiology 1:25, 1990.
Nishikawa et al J. Biol Chem. 263; 8270-8281, 1988.
M. G. Shoreibah et al J. Biol. Chem. 267: 2920-2927:1992.
Schanbacher and Ebner, J. Biol. Chem. 245:5057-5061, 1970.
Yoon and Laine, Glycobiology 2:161-168, 1992.
Holt, G. D. et al J. Cell. Biol. 104:1157, 1987.
Viitala, J. and Finne, J. Eur. J. Biochem. 138:393, 1984
Cornil, I. et al, J. Cell Biol. 111:773, 1990.
Williams and Schachter, (1980) J. Biol. Chem. 255:11247.
Williams et al (1980) J. Biol. Chem. 255:11253.
Schacter et al. (1971) J. Biol. Chem. 246:5321.
Greer et al., Biochem. Cell Biol., vol. 67, pp. 503-509, 1989.
Thrope et al., Development, 102:193-210, 1988.
Datti Alessandro
Dennis James W.
Siminovitch Katherine A.
Leary Louise N.
Mount Sinai Hospital Corporation
Wityshyn Michael G.
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