Chemistry: electrical and wave energy – Processes and products – Processes of treating materials by wave energy
Patent
1993-09-28
1995-04-25
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Processes of treating materials by wave energy
2041531, G01N 2726
Patent
active
054095832
ABSTRACT:
The present invention provides a method for measuring concentrations of the first and second substrates in a sample liquid by using a biosensor. The biosensor has an electrically insulating substrate, an electrode system, and a reaction layer including a first enzyme, a second enzyme and an electron acceptor. The method includes the steps of measuring a response current value I.sub.1 of a predetermined period of time T.sub.1 after supplying the sample liquid including the first and second substrates to the biosensor; measuring a response current value I.sub.2 of a predetermined period of time T.sub.2 after the supply of the sample liquid; obtaining the concentration C.sub.1 of the first substrate corresponding to the current value I.sub.1 based on the previously determined relationship between the concentration of the first substrate and the response current value of the predetermined period of time T.sub.1 after the supply; obtaining a response current value I.sub.1 ' corresponding to the concentration C.sub.1 of the first substrate in the sample liquid based on the previously determined relationship between the concentration of the first substrate and the response current value of the predetermined period of time T.sub.2 after the supply; and obtaining the concentration C.sub.2 of the second substrate in the sample liquid corresponding to the current value calculated by subtracting the current value I.sub.1 ' from the current value I.sub.2 based on the previously determined relationship between the concentration of the second substrate and the response current value of the predetermined period of time T.sub.2 after the supply.
REFERENCES:
patent: 5135876 (1992-08-01), Andrade
patent: 5192415 (1993-03-01), Yoshioka et al.
patent: 5264103 (1993-11-01), Yoshioka et al.
patent: 5264103 (1993-11-01), Yoshioka et al.
Search Report for European Appln. 931/5800.0, Mailed Jan. 12, 1994.
Fujisawa Satoko
Miyahara Mariko
Nankai Shiro
Yoshioka Toshihiko
Bell Bruce F.
Matsushita Electric - Industrial Co., Ltd.
Niebling John
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