Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving oxidoreductase
Patent
1994-03-18
1996-08-20
Paden, Carolyn
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving oxidoreductase
435822, 435823, 435 25, 435 4, C12Q 125
Patent
active
055478514
ABSTRACT:
A method for measuring carbon dioxide, comprising the steps of: (1) reacting bicarbonate ion in a sample with phosphoenolpyruvate carboxylase derived from an acetic acid bacterium in the presence of phosphoenolpyruvate; (2) reacting the resultant oxalacetic acid with malate dehydrogenase in the presence of NADH; and (3) measuring decreased NADH, and a reagent for measuring carbon dioxide, comprising phosphoenolpyruvate, phosphoenolpyruvate carboxylase derived from an acetic acid bacterium, malate dehydrogenase, NADH and a buffer. According to the present invention, a highly stable reagent for CO.sub.2 measurement, permitting a long-term storage in a liquid state can be provided by the use of phosphoenolpyruvate carboxylase derived from an acetic acid bacterium.
REFERENCES:
patent: 3974037 (1976-08-01), Adams
Gossele et al. System. Appl. Microbiol. vol. 4, pp. 338-368 (1983).
Benziman, et al. J. Bacteriology vol. 98, No. 3, pp. 1005-1010 (1969), "Role of Phosphoenolpyruvate Carboxylation in Acetobacter xylinum".
Schwitzguebel et al., "Phosphenolpyruvate Carboxylase from Acetobacter aceti", Arch. Microbiol, 122, 109-115 (1979).
Emi Shigenori
Hattori Shizuo
Kishimoto Takahide
Sogabe Yukihiro
Paden Carolyn
Toyo Boseki Kabushiki Kaisha
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