Method for measuring carbon dioxide and reagent therefor

Details Method for measuring carbon dioxide and reagent therefor Method for measuring carbon dioxide and reagent therefor

1995-06-06

1997-04-29

Pratt, Helen

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving oxidoreductase

435823, 422 84, C12Q 125, G01N 3350

Patent

active

056248121

ABSTRACT:
A method for measuring carbon dioxide, comprising the steps of: (1) reacting bicarbonate ion in a sample with phosphoenolpyruvate carboxylase derived from an acetic acid bacterium in the presence of phosphoenolpyruvate; (2) reacting the resultant oxalacetic acid with malate dehydrogenase in the presence of NADH; and (3) measuring decreased NADH, and a reagent for measuring carbon dioxide, comprising phosphoenolpyruvate, phosphoenolpyruvate carboxylase derived from an acetic acid bacterium, malate dehydrogenase, NADH and a buffer. According to the present invention, a highly stable reagent for CO.sub.2 measurement, which permits a long-term storage in a liquid state, can be provided by the use of phosphoenolpyruvate carboxylase derived from a species of acetic acid bacteria.

REFERENCES:
patent: 3963578 (1976-06-01), Aitken et al.
Tietz, et al, 1986, Clinical Chemistry, W.B. Saunders Co., London, pp. 1188-1189, 1190.

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