Chemistry: analytical and immunological testing – Automated chemical analysis – With a continuously flowing sample or carrier stream
Patent
1995-02-24
1996-12-17
Housel, James C.
Chemistry: analytical and immunological testing
Automated chemical analysis
With a continuously flowing sample or carrier stream
422 81, 436172, 436815, G01N 3508
Patent
active
055852742
ABSTRACT:
A method for measuring .beta.-(1,3)(1,4)-D-glucan in a sample wherein a reaction solution containing calcofluor and a sample containing .beta.-(1,3)(1,4)-D-glucan are introduced in a reaction zone of a flow injection system and wherein a constant temperature of 0.degree. to 40.degree. C. is maintained at least between a site at which the reactant solution containing calcofluor is mixed with the sample containing .beta.-(1,3)(1,4)-D-glucan, and a detector. When the amount of sample injected into the system is between 0.002 ml and 0.1 ml, the relationship between the void volume in the reaction zone and the amount of sample introduced into the system is determined as follows: when the void volume is less than 0.4 ml: 0.03.times.(void volume (ml))+0.0018.ltoreq.amount of sample (ml).ltoreq.0.066.times.(void volume (ml))+0.0018; when the void volume is 0.4 ml or more: 0.01.times.(void volume (ml))+0.0098.ltoreq.amount of sample (ml).ltoreq.0.026.times.(void volume (ml))+0.00178. The present invention results in an accurate and reproducible measurement of the .beta.-(1,3)(1,4)-D-glucan content in a sample, even if saccharides and ethanol are present. Therefore, using the method of the present invention, it is possible to objectively compare and evaluate the .beta.-(1,3)(1,4)-D-glucan contents in various samples, such as cereals, malt extract, wort, beer, etc.
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Freed Rachel
Housel James C.
Sapporo Breweries Limited
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